This antibody stains schwannoma, ependymomas, astrogliomas, almost all benign and malignant melanoma and their metasis. Cellular Localization: Cytoplasmic.
Cross-Reactivity (Details)
Species reactivity (tested):Human, Monkey, Bovine, Rat and Mouse
Characteristics
Synonyms: S100 Calcium binding protein, S-100 protein, S100A, S100B, S-100 protein alpha chain,S-100 protein beta chain, Astrocyte Marker, S-100 protein alpha subunit, S-100 proteinbeta subunit, S100 Alpha, S100 Beta
S100A1
Reactivity: Human, Mouse, Rat
WB, ELISA, IHC, IF
Host: Rabbit
Polyclonal
unconjugated
Application Notes
Suitable for Immunohistochemistry and Immunocytochemistry (Frozen or Formalin-FixedParaffin-Embedded (FFPE) tissue sections and cell smears)For IHC dilute concentared antibody at 1/50-1/100, use streptavidinapprox. biotin system orpolymer system, incubate 30 minutes at room temperature.
Restrictions
For Research Use only
Concentration
0.2 mg/mL
Buffer
PBS, pH 7.4, 0.05 % Sodium Azide, 1 % BSA
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light. Shelf life: One year from despatch.
S100 protein is a type of low molecular weight protein found in vertebrates characterized by two calcium binding sites of the helix-loop-helix ("EF-hand type") conformation. There are at least 21 different types of S100 proteins. The name is derived from the fact that the protein is 100 % Soluble in ammonium sulfate at neutral pH . Most S100 proteins are homodimeric, consisting of two identical polypeptides held together by non-covalent bonds. S100A is composed of an alpha and beta chain whereas S100B is composed of two beta chains. Although S100 proteins are structurally similar to calmodulin, they differ in that they are cell-specific, expressed in particular cells at different levels depending on environmental factors. To contrast, calmodulin is a ubiquitous and universal intracellular Ca++ receptor widely expressed in many cells.S-100 is normally present in cells derived from the neural crest (Schwann cells, melanocytes, and glia cells), chondrocyte, adipocytes, myoepithelial cells, macrophages, Langerhans cells, dendritic cells, and keratinocytes. It may be present in some breast epithelial cells.Synonyms: Astrocyte Marker, S-100 protein, S-100 protein alpha chain, S-100 protein alpha subunit, S-100 protein beta chain, S-100 protein beta subunit, S100 Alpha, S100 Beta, S100 Calcium binding protein, S100A, S100B