ERK1/2 antibody
-
- Target See all ERK1/2 (MAPK1/3) Antibodies
- ERK1/2 (MAPK1/3) (Mitogen-Activated Protein Kinase 1/3 (MAPK1/3))
-
Reactivity
- Human, Mouse, Rat, Hamster
-
Host
- Rabbit
-
Clonality
- Polyclonal
-
Conjugate
- This ERK1/2 antibody is un-conjugated
-
Application
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunocytochemistry (ICC)
- Specificity
- Detects endogenous levels of total p44/42 MAP kinase (Erk1/Erk2) protein. In some cell types, recognizes p42 MAPK more readily than p44 MAPK. Does not recognize JNK/SAPK nor p38 MAP kinase. Species cross-reactivity: Human, mouse, rat, hamster, zebrafish.
- Purification
- Immunoaffinity purified
- Immunogen
-
Synthetic peptide (KLH coupled) derived from the sequence of rat p42 MAP kinase.
Type of Immunogen: Synthetic peptide - Isotype
- IgG
- Top Product
- Discover our top product MAPK1/3 Primary Antibody
-
-
- Application Notes
-
Approved: ELISA, Flo (1:25), ICC, IF, IHC, IHC-P, IP (1:50), WB (1:1000)
Usage: Suitable for use in Immunofluorescence, Immunocytochemistry, ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry and Flow Cytometry. Western Blot: 1:1000, Incubate membrane with diluted antibody in 5 % BSA, 1X TBS, 0.1 % Tween-20 at 4?C with gentle shaking, overnight. Immunohistochemistry (Paraffin sections): 1:100. Immunoprecipitation: 1:50. Immunocytochemistry (IF): 1:25. Flow Cytometry: 1:25. - Comment
-
Target Species of Antibody: Rat
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- 10 mM HEPES, pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol. No preservative added.
- Preservative
- Without preservative
- Handling Advice
- Avoid repeat freeze-thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
-
- Target
- ERK1/2 (MAPK1/3) (Mitogen-Activated Protein Kinase 1/3 (MAPK1/3))
- Alternative Name
- ERK1 + ERK2 (MAPK1/3 Products)
- Synonyms
- erk1/2 antibody, mitogen-activated protein kinase antibody, erk1/2 antibody
- Background
-
Name/Gene ID: ERK1/ERK2
Synonyms: ERK1/ERK2
This antibody detects: ERK1 + ERK2
-