ERCC3 antibody (AA 242-261)
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- Target See all ERCC3 Antibodies
- ERCC3 (DNA Repair Protein Complementing XP-B Cells (ERCC3))
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Binding Specificity
- AA 242-261
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This ERCC3 antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Brand
- IHC-plus™
- Specificity
- Recognizes an epitope located between aa242-261 of ERCC-3 (largest subunit of TFIIH).
- Purification
- Protein G purified
- Immunogen
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Synthetic peptide corresponding to aa242-261 of human ERCC-3.
Type of Immunogen: Synthetic peptide - Clone
- 3G4
- Isotype
- IgG2b
- Top Product
- Discover our top product ERCC3 Primary Antibody
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- Application Notes
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Approved: ELISA, IHC, IHC-P (5 - 10 μg/mL), IP, WB
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5-10 μg/mL. Immunoprecipitation: Immunoprecipitates transcriptionally active TFIIH in a reconstituted assay. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- 0.1 M Tris-HCl, pH 7.5. Sourced from ascites.
- Handling Advice
- Avoid repeat freeze-thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- Target
- ERCC3 (DNA Repair Protein Complementing XP-B Cells (ERCC3))
- Alternative Name
- ERCC3 / XPB (ERCC3 Products)
- Background
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Name/Gene ID: ERCC3
Synonyms: ERCC3, BTF2, GTF2H, RAD25, TFIIH p89, TFIIH, XPB, BTF2 p89, XPBC, TFIIH 89 kDa subunit - Gene ID
- 2071
- UniProt
- P19447
- Pathways
- DNA Damage Repair
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