ERCC3 antibody (AA 242-261)

Catalog No. ABIN351537

Product Details anti-ERCC3 Antibody

Target: ERCC3 (DNA Repair Protein Complementing XP-B Cells (ERCC3))

Binding Specificity: AA 242-261

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ERCC3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Recognizes an epitope located between aa242-261 of ERCC-3 (largest subunit of TFIIH).

Purification: Protein G purified

Immunogen: Synthetic peptide corresponding to aa242-261 of human ERCC-3.

Type of Immunogen: Synthetic peptide

Clone: 3G4

Isotype: IgG2b

Application Details

Application Notes: Approved: ELISA, IHC, IHC-P (5 - 10 μg/mL), IP, WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5-10 μg/mL. Immunoprecipitation: Immunoprecipitates transcriptionally active TFIIH in a reconstituted assay.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.1 M Tris-HCl,  pH 7.5. Sourced from ascites.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for ERCC3

Target: ERCC3 (DNA Repair Protein Complementing XP-B Cells (ERCC3))

Alternative Name: ERCC3 / XPB (ERCC3 Products)

Synonyms: BTF2 antibody, GTF2H antibody, RAD25 antibody, TFIIH antibody, XPB antibody, BTF2 p89 antibody, Ercc-3 antibody, wu:fc25f08 antibody, ERCC excision repair 3, TFIIH core complex helicase subunit antibody, excision repair cross-complementing rodent repair deficiency, complementation group 3 antibody, excision repair cross-complementation group 3 antibody, ERCC3 antibody, Ercc3 antibody, ercc3 antibody

Background: Name/Gene ID: ERCC3

Synonyms: ERCC3, BTF2, GTF2H, RAD25, TFIIH p89, TFIIH, XPB, BTF2 p89, XPBC, TFIIH 89 kDa subunit

Gene ID: 2071

UniProt: P19447

Pathways: DNA Damage Repair