PF4 antibody

Catalog No. ABIN351496

Product Details anti-PF4 Antibody

Target: PF4 (Platelet Factor 4 (PF4))

Reactivity: Human

Host: Sheep

Clonality: Polyclonal

Conjugate: This PF4 antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Recognizes human PF4.

Purification: Immunoaffinity purified

Immunogen: Platelet Factor 4 (PF4) purified from human platelet releasate.

Isotype: IgG

Application Details

Application Notes: Approved: ELISA, IHC, IHC-P (5 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.01 M HEPES,  pH 7.4, 0.15 M sodium chloride, 50 % glycerol. No preservative added.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

References for anti-PF4 antibody (ABIN351496)

Ihnatko, Truchla, Ihnatková, Prohászka, Lazúrová: "Case Report: A Case of COVID Vaccine-Induced Thrombotic Thrombocytopenia Manifested as Pulmonary Embolism and Hemorrhagia. A First Reported Case From Slovakia." in: Frontiers in medicine, Vol. 8, pp. 789972, (2021) (PubMed).

Target Details for PF4

Target: PF4 (Platelet Factor 4 (PF4))

Alternative Name: CXCL4 / PF4 (PF4 Products)

Synonyms: CXCL4 antibody, PF-4 antibody, SCYB4 antibody, Cxcl4 antibody, Scyb4 antibody, Pf4a antibody, RATPF4A antibody, PF4A antibody, CXCL4L1 antibody, CXCL4V1 antibody, PF4-ALT antibody, SCYB4V1 antibody, PF4 antibody, platelet factor 4 antibody, platelet factor 4 variant 1 antibody, PF4 antibody, Pf4 antibody, LOC703451 antibody, PF4V1 antibody, LOC100524373 antibody, LOC102181854 antibody, LOC100630489 antibody

Background: Name/Gene ID: PF4
Subfamily: Intercrine alpha
Family: Intercrine

Synonyms: PF4, C-X-C motif chemokine 4, CXCL4, Iroplact, Oncostatin-A, PF-4, SCYB4, Platelet factor 4

Gene ID: 5196

UniProt: P02776