NOD2 antibody (C-Term)

Catalog No. ABIN351444

Product Details anti-NOD2 Antibody

Target: NOD2 (Nucleotide-Binding Oligomerization Domain Containing 2 (NOD2))

Binding Specificity: C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NOD2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: NOD2 has no cross-reaction with NOD1.

Purification: Immunoaffinity purified

Isotype: IgG

Application Details

Application Notes: Approved: ELISA, ICC (10 μg/mL), IHC, IHC-P (3 μg/mL), WB (2 - 4 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 3 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles. Store undiluted.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for 3 months and -20°C, stable for up to 1 year. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for NOD2

Target: NOD2 (Nucleotide-Binding Oligomerization Domain Containing 2 (NOD2))

Alternative Name: NOD2 / CARD15 (NOD2 Products)

Background: Name/Gene ID: NOD2

Synonyms: NOD2, BLAU, CARD15, ACUG, NOD-like receptor C2, NOD2B, NLRC2, IBD1, CLR16.3, PSORAS1

Gene ID: 64127

UniProt: Q9HC29

Pathways: Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Toll-Like Receptors Cascades, Inflammasome