VAMP2 antibody (AA 10-60)

Catalog No. ABIN351358

Product Details anti-VAMP2 Antibody

Target: VAMP2 (Vesicle-Associated Membrane Protein 2 (Synaptobrevin 2) (VAMP2))

Binding Specificity: AA 10-60

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This VAMP2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Specificity: Specific for VAMP2 and VAMP1.

Cross-Reactivity: Human, Mouse, Rat

Cross-Reactivity (Details): Other species not yet tested.

Purification: IgG

Immunogen: A synthetic peptide from AA 10-60 of human VAMP2 conjugated to blue carrier protein was used as the antigen. The VAMP2 peptide shares 93% shared identity with VAMP1. The peptide is homologous in many other species including rat, mouse and xenopus.

Isotype: IgG

Application Details

Application Notes: IHC, WB. A working concentration of 10-50 μg,ml is recommended. The optimal concentration should be determined by the end user. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in 500 μL of sterile water. Centrifuge to remove any insoluble material.

Handling Advice: Avoid freeze and thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date: 12 months

Target Details for VAMP2

Target: VAMP2 (Vesicle-Associated Membrane Protein 2 (Synaptobrevin 2) (VAMP2))

Alternative Name: VAMP 2 (VAMP2 Products)

Synonyms: zgc:73302 antibody, wu:fj61c06 antibody, VAMP2 antibody, vamp2-a antibody, xsybi antibody, Syb-2 antibody, Syb2 antibody, sybII antibody, RATVAMPB antibody, RATVAMPIR antibody, SYB antibody, SYB2 antibody, VAMP-2 antibody, syb2 antibody, vamp2 antibody, xsybii antibody, vesicle-associated membrane protein 2 antibody, vesicle associated membrane protein 2 antibody, vesicle associated membrane protein 2 S homeolog antibody, vesicle-associated membrane protein 2 (synaptobrevin 2) antibody, vesicle associated membrane protein 2 L homeolog antibody, vamp2 antibody, VAMP2 antibody, vamp2.S antibody, Vamp2 antibody, vamp2.L antibody

Background: The protein encoded by this gene is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. Synaptobrevins/VAMPs, syntaxins, and the 25-kD synaptosomal-associated protein SNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. This gene is thought to participate in neurotransmitter release at a step between docking and fusion. The protein forms a stable complex with syntaxin, synaptosomal-associated protein, 25 kD, and synaptotagmin. It also forms a distinct complex with synaptophysin. It is a likely candidate gene for familial infantile myasthenia (FIMG) because of its map location and because it encodes a synaptic vesicle protein of the type that has been implicated in the pathogenesis of FIMG. FUNCTION: Involved in the targeting and/or fusion of transport vesicles to their target membrane. SUBCELLULAR LOCATION: Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane, Single-pass type IV membrane protein. Cell junction, synapse, synaptosome. Note: Neuronal synaptic vesicles.,Vesicle Transport,VAMP-2, Synaptobrevin-2, VAMP2, SYB2. VAMP1, Synaptobrevin-1

UniProt: P63027

Pathways: Peptide Hormone Metabolism, Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport