RHOA antibody (AA 100-150)

Catalog No. ABIN351037

Product Details anti-RHOA Antibody

Target: RHOA (Ras Homolog Gene Family, Member A (RHOA))

Binding Specificity: AA 100-150

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RHOA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Specificity: Specific for RhOA.

Cross-Reactivity: Human, Marmoset, Mouse, Rat

Cross-Reactivity (Details): Other species not yet tested.

Purification: IgG

Immunogen: A synthetic peptide from AA 100-150 of human RhoA conjugated to blue carrier protein was used as the antigen. The peptide is homologous in many species including rat, mouse, chicken, dog, bovine, frog, zebrafish and orangutan.

Isotype: IgG

Application Details

Application Notes: IHC, WB. A concentration of 10-50 μg,ml is recommended. The optimal concentration should be determined by the end user.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in 500 μL of sterile water. Centrifuge to remove any insoluble material.

Handling Advice: Avoid freeze and thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date: 12 months

Target Details for RHOA

Target: RHOA (Ras Homolog Gene Family, Member A (RHOA))

Alternative Name: RhoA (RHOA Products)

Background: FUNCTION: Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. May be an activator of PLCE1. SUBUNIT: Binds PRKCL1, ROCK1 and ROCK2. Interacts with ARHGEF3, NET1 and RTKN. Interacts with PLCE1 and AKAP13. Interacts with RGNEF. SUBCELLULAR LOCATION: Cell membrane, Lipid-anchor, Cytoplasmic side. Cytoplasm, cytoskeleton.,Rho Family,Arha, Arha2, ras homolog gene family member A, MGC72339

UniProt: P61586

Pathways: Microtubule Dynamics, WNT Signaling, Neurotrophin Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Actin Filament Polymerization, Cell-Cell Junction Organization, Positive Regulation of Endopeptidase Activity, Signaling Events mediated by VEGFR1 and VEGFR2, Thromboxane A2 Receptor Signaling, SARS-CoV-2 Protein Interactome