A synthetic peptide from AA 100-144 of human SOD1 conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat and mouse.
SOD1
Reactivity: Human, Mouse, Rat
WB, ELISA, ICC, IHC (p), FACS, IHC (fro), IF (p), IF (cc)
Host: Rabbit
Polyclonal
unconjugated
Application Notes
IHC, WB (confirmed by recombinant protein). A concentration of 10-50 μg,ml is recommended. The optimal concentration should be determined by the end user. Not yet tested in other applications.
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Reconstitute in 500 μL of sterile water. Centrifuge to remove any insoluble material.
Handling Advice
Avoid freeze and thaw cycles.
Storage
4 °C/-20 °C
Storage Comment
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Function: Destroys radicals which are normally produced within the cells and which are toxic to biological systems. Catalytic activity: 2 superoxide + 2 H(+) = O(2) + H(2)O(2). Subcellular location: Cytoplasm. Involvement in disease: Defects in SOD1 are the cause of amyotrophic lateral sclerosis type 1 (ALS1). ALS1 is a familial form of amyotrophic lateral sclerosis, a neurodegenerative disorder affecting upper and lower motor neurons and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology of amyotrophic lateral sclerosis is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10 % of cases leading to familial forms. The protein (both wild-type and ALS1 variants) has a tendency to form fibrillar aggregates in the absence of the intramolecular disulfide bond or of bound zinc ions. These aggregates may have cytotoxic effects. Zinc binding promotes dimerization and stabilizes the native form.,Signal Transduction,SOD1, Superoxide dismutase 1, SODC