SQSTM1 antibody (AA 300-360)

Catalog No. ABIN350829

Product Details anti-SQSTM1 Antibody

Target: SQSTM1 (Sequestosome 1 (SQSTM1))

Binding Specificity: AA 300-360

Reactivity: Human

Host: Sheep

Clonality: Polyclonal

Conjugate: This SQSTM1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: Specific for sequestosome-1.

Cross-Reactivity: Human

Cross-Reactivity (Details): Other species not yet tested.

Purification: IgG

Immunogen: A synthetic peptide from AA 300-360 of human SQSTM1 has been used as the antigen.

Isotype: IgG

Application Details

Application Notes: IHC (both on frozen,paraffin embedded tissues), WB. Use at a concentration of 10-50 μg,ml. The optimal concentration should be determined by the end user.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in 500 μL of sterile water. Centrifuge to remove any insoluble material.

Handling Advice: Avoid freeze and thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date: 12 months

Target Details for SQSTM1

Target: SQSTM1 (Sequestosome 1 (SQSTM1))

Alternative Name: SQSTM1 (SQSTM1 Products)

Synonyms: SQSTM1 antibody, sqstm1 antibody, MGC79491 antibody, A170 antibody, OSIL antibody, PDB3 antibody, ZIP3 antibody, p60 antibody, p62 antibody, p62B antibody, OSF-6 antibody, Osi antibody, STAP antibody, ZIP antibody, sb:cb621 antibody, zgc:85784 antibody, sequestosome 1 antibody, sequestosome 1 L homeolog antibody, SQSTM1 antibody, sqstm1 antibody, Sqstm1 antibody, sqstm1.L antibody

Background: FUNCTION: Adapter protein which binds ubiquitin and may regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1. May play a role in titin/TTN downstream signaling in muscle cells. May regulate signaling cascades through ubiquitination. May be involved in cell differentiation, apoptosis, immune response and regulation of K(+) channels. SUBCELLULAR LOCATION: Cytoplasm. Late endosome. Nucleus. Note=Sarcomere. In cardiac muscles localizes to the sarcomeric band. Localizes to late endosomes. May also localize to the nucleus. Accumulates in neurofibrillary tangles and in Lewy bodies of neurons from individuals with Alzheimer and Parkinson disease respectively. Enriched in Rosenthal fibers of pilocytic astrocytoma. In liver cells, accumulates in Mallory bodies associated with alcoholic hepatitis, Wilson disease, indian childhood cirrhosis and in hyaline bodies associated with hepatocellular carcinoma. TISSUE SPECIFICITY: Ubiquitously expressed.,Ubiquitination,Sequestosome 1, phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa, ubiquitin-binding protein p62, EBI3-associated protein of 60 kDa, p60, EBIAP, SQSTM1, A170, OSIL, PDB3, ZIP3, p62B

UniProt: Q13501

Pathways: NF-kappaB Signaling, Neurotrophin Signaling Pathway, Autophagy