A synthetic peptide as a part of human, rat, mouse Rho-related GTP-binding protein RhoB (ARH6, ARHB, H6) conjugated to an immunogenic carrier protein was used as the immunogen.
RHOB
Reactivity: Human
WB, IP
Host: Rabbit
Polyclonal
unconjugated
Application Notes
IHC, WB. A concentration of 10-50 μg,ml is recommended. The optimal concentration should be determined by the end user.
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Reconstitute in 500 μL of sterile water. Centrifuge to remove any insoluble material.
Handling Advice
Avoid freeze and thaw cycles.
Storage
4 °C/-20 °C
Storage Comment
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
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Background
FUNCTION: Mediates apoptosis in neoplastically transformed cells after DNA damage. Not essential for development but affects cell adhesion and growth factor signaling in transformed cells. Plays a negative role in tumorigenesis as deletion causes tumor formation. Involved in intracellular protein trafficking of a number of proteins. Targets PKN1 to endosomes and is involved in trafficking of the EGF receptor from late endosomes to lysosomes. Also required for stability and nuclear trafficking of AKT1/AKT which promotes endothelial cell survival during vascular development. SUBUNIT: Binds ROCK1 and ROCK2. Also binds PKN1/PRK1. Interacts with ARHGEF3 and AKAP13. Interacts with RTKN. SUBCELLULAR LOCATION: Late endosome membrane, Lipid-anchor. Cell membrane, Lipid-anchor. Nucleus. Note: Late endosomal membrane (geranylgeranylated form). Plasma membrane (farnesylated form). Also detected at the nuclear margin and in the nucleus. INDUCTION: By UV irradiation, N-methyl-N-nitrosourea, cisplatin, cyclohexamide and serum stimulation.,Rho Family,H6, ARH6, ARHB, RHOH6, MST081, MSTP081