NGFR antibody (Extracellular Domain)

Catalog No. ABIN350712

Product Details anti-NGFR Antibody

Target: NGFR (Nerve Growth Factor Receptor (NGFR))

Binding Specificity: Extracellular Domain

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NGFR antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Specificity: Specific for p75NTR.

Cross-Reactivity: Human, Mouse, Rat

Cross-Reactivity (Details): Other species not yet tested.

Purification: IgG

Immunogen: A synthetic peptide from the extracellular domain of human p75NTR (low affinity NGF receptor, p75, LNGFR, p75NTR) conjugated to an immunogenic carrier protein was used as the antigen. The antigen is homologous in many other species including mouse and rat.

Isotype: IgG

Application Details

Application Notes: IHC, WB. A concentration of 10-50 μg,ml is recommended. The optimal concentration should be determined by the end user. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in 500 μL of sterile water. Centrifuge to remove any insoluble material.

Handling Advice: Avoid freeze and thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date: 12 months

Target Details for NGFR

Target: NGFR (Nerve Growth Factor Receptor (NGFR))

Alternative Name: p75NTR (NGFR Products)

Background: FUNCTION: Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells. SUBUNIT: Homodimer, disulfide-linked. Interacts with p75NTR-associated cell death executor. Interacts with NGFRAP1/<||a href="http://www.osenses.com/bex-family-c-21_106.html">BEX3<||/a>. Interacts with TRAF2, TRAF4, TRAF6, PTPN13 and RANBP9. Interacts through TRAF6 with <||a href="http://www.osenses.com/advanced_search_result.php?antigen=sqstm1&x=0&y=0">SQSTM1<||/a> which bridges NGFR to NTRK1. Interacts with <||a href="http://www.osenses.com/bex-family-c-21_106.html">BEX1<||/a>. Interacts with ARMS and NTRK1. Can form a ternary complex with NTRK1 and ARMS and this complex is affected by the expression levels of ARMS. An increase in ARMS expression leads to a decreased association of NGFR and NTRK1. Interacts with LINGO1. SUBCELLULAR LOCATION: Membrane, Single-pass type I membrane protein.,Neurotrophic Receptors,Low-affinity nerve growth factor receptor, NGF receptor, Gp80-LNGFR, p75 ICD, Low affinity neurotrophin receptor p75NTR, p75, LNGFR, p75NTR, RNNGFRR

UniProt: P08138

Pathways: NF-kappaB Signaling, Neurotrophin Signaling Pathway, Carbohydrate Homeostasis, Growth Factor Binding