GAPDH antibody (C-Term)
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- Target See all GAPDH Antibodies
- GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))
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Binding Specificity
- C-Term
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Reactivity
- Rat
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Host
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Sheep
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Clonality
- Polyclonal
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Conjugate
- This GAPDH antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC)
- Specificity
- Specific for GAPDH.
- Cross-Reactivity
- Human, Mouse, Rat
- Cross-Reactivity (Details)
- Other species not yet tested.
- Purification
- IgG
- Immunogen
- A synthetic peptide from c-terminal of rat GAPDH conjugated to blue carrier protein was used as the antigen. The antigen is homologous in mouse and shares 90% identity with human sequence.
- Isotype
- IgG
- Top Product
- Discover our top product GAPDH Primary Antibody
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anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) antibody
GAPDH Reactivity: Human WB, IF, IHC (p), IP, ICC Host: Rabbit Polyclonal unconjugated
anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 62-91), (N-Term) antibodyGAPDH Reactivity: Human WB, IF, IHC (p) Host: Rabbit Polyclonal RB16537 unconjugated
anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 1-335) antibodyGAPDH Reactivity: Human WB, IHC, IF, IP Host: Rabbit Polyclonal unconjugated
anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) antibodyGAPDH Reactivity: Human, Mouse, Rat WB, IF, IHC (p) Host: Mouse Monoclonal 1A10A10 unconjugated
anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (C-Term) antibodyVerified GAPDH Reactivity: Human, Rat WB, ELISA, IHC, IF Host: Goat Polyclonal unconjugated
anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 233-259), (C-Term) antibodyGAPDH Reactivity: Human WB, IF, IHC (p), FACS Host: Rabbit Polyclonal RB16543 unconjugated
anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (Internal Region) antibodyVerified GAPDH Reactivity: Human, Mouse, Rat WB, ELISA, IHC, IF Host: Goat Polyclonal unconjugated
anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 226-335) antibodyGAPDH Reactivity: Human WB, ELISA Host: Mouse Monoclonal 3C2 unconjugated
anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 136-335) antibodyGAPDH Reactivity: Human, Mouse, Rat WB, IHC (p) Host: Rabbit Polyclonal unconjugated
anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 3-335) antibodyGAPDH Reactivity: Human WB, ELISA, IHC, IF Host: Rabbit Polyclonal unconjugated
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- Application Notes
- IHC, WB. A concentration of 10-50 μg,ml is recommended. The optimal concentration should be determined by the end user. Not yet tested in other applications.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Reconstitute in 500 μL of sterile water. Centrifuge to remove any insoluble material.
- Handling Advice
- Avoid freeze and thaw cycles.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
- Expiry Date
- 12 months
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- Target
- GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))
- Alternative Name
- GAPDH (GAPDH Products)
- Background
- FUNCTION: May bind tRNA. Has uracil-DNA-glycosylase activity. May activate transcription in neurons. Functions as a killing protein in apoptosis when over-expressed. CATALYTIC ACTIVITY: D-glyceraldehyde 3-phosphate + phosphate + NAD+ = 3-phospho-D-glyceroyl phosphate + NADH. PATHWAY: Carbohydrate degradation, glycolysis, pyruvate from D-glyceraldehyde 3-phosphate: step 1/5. SUBUNIT: Homotetramer. SUBCELLULAR LOCATION: Cytoplasm. TISSUE SPECIFICITY: High levels in skeletal muscle and heart, low levels in liver, brain, and kidney.,Neurodegeneration,38 kDa BFA dependent ADP ribosylation substrate, BARS-38, Glyceraldehyde-3-phosphate dehydrogenase
- UniProt
- P04797
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