GFP antibody

Catalog No. ABIN350302

Product Details anti-GFP Antibody

Target: GFP (Green Fluorescent Protein (GFP))

Reactivity: Aequorea victoria

Host: Sheep

Clonality: Polyclonal

Conjugate: This GFP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Specificity: Specific for EGFP/GFP/Venus

Immunogen: Synthetic peptides (a pentamer) from EGFP/GFP/Venus conjugated to an immunogenic carrier protein was used as the antigen.

Isotype: IgG

Application Details

Application Notes: Use at a concentration of 10-50 µg/ml.
The optimal working concentration should be determined by the end user.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in 100 µL of MQ water. Centrifuge to remove any insoluble material.

Handling Advice: Avoid freeze and thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date: 12 months

References for anti-GFP antibody (ABIN350302)

Choi, Kim, Song, Cha, Cho, Son, Kim, Mook-Jung: "Amyloid β-induced elevation of O-GlcNAcylated c-Fos promotes neuronal cell death." in: Aging cell, Vol. 18, Issue 1, pp. e12872, (2020) (PubMed).

Target Details for GFP

Target: GFP (Green Fluorescent Protein (GFP))

Abstract: GFP Products

Synonyms: green fluorescent protein antibody, gfp antibody

Background: GFP is a  naturally fluorescent protein, discovered by chance in the 1960s by Shimomura et al. GFP makes green light out of aequorin’s blue light. The chromophore is a modified Ser-Tyr-Gly sequence. GFP can emit light once this short tripeptide has adopted a cyclic conformation with the help of a oxygen. BIOPHYSICOCHEMICAL PROPERTIES: Excitation max (nm): 488, Emission max (nm): 509, Extinction coefficient (Cm-1M-1): 61000. Fluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions. Also known as: Enhanced Green Fluorescence Protein.