Choline Acetyltransferase antibody (AA 590-640)

Catalog No. ABIN350213

Product Details anti-Choline Acetyltransferase Antibody

Target: Choline Acetyltransferase (CHAT) (Choline O-Acetyltransferase (CHAT))

Binding Specificity: AA 590-640

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Choline Acetyltransferase antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Specificity: Specific for ChAT.

Cross-Reactivity (Details): May contain small amounts of intact IgG

Purity: > 90 % based on SDS-PAGE

Immunogen: A synthetic peptide from aa regio 590-640 of mouse ChAT conjugated to an immunogenic carrier protein was used as the antigen.

Isotype: IgG

Application Details

Application Notes: A concentration of 10-50 µg/ml is recommended.
The optimal concentration should be determined by the end user.
Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.

Handling Advice: Avoid freeze and thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date: 12 months

References for anti-Choline Acetyltransferase antibody (ABIN350213)

Wang, Wu, Wu, Sun, Zhang, Deng, Walker, Qu, Chen, Liu, Han, Dai, Shields, Shields, Sengelaub, Jones, Smith, Xu: "Remodeling of lumbar motor circuitry remote to a thoracic spinal cord injury promotes locomotor recovery." in: eLife, Vol. 7, (2019) (PubMed).

Target Details for Choline Acetyltransferase

Target: Choline Acetyltransferase (CHAT) (Choline O-Acetyltransferase (CHAT))

Alternative Name: ChAT (CHAT Products)

Synonyms: CHOACTASE antibody, CMS1A antibody, CMS1A2 antibody, ChoAcTase antibody, B230380D24Rik antibody, choline O-acetyltransferase antibody, choline O-acetyltransferase a antibody, choline acetyltransferase antibody, carnitine O-acetyltransferase antibody, CHAT antibody, chata antibody, Chat antibody, PGTG_00551 antibody, LOC100533322 antibody

Background: Cholinergic systems are implicated in numerous neurologic functions. Alteration in some cholinergic neurons may account for the disturbances of Alzheimer disease. The protein encoded by this gene synthesizes the neurotransmitter acetylcholine. Alternative splice variants have been found that contain alternative 5' untranslated exons. Three of the four described splice variants encode identical 69 kDa proteins while one variant encodes both the 69 kDa and a larger 82 kDa protein. Choline acetylase  catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.
Catalytic activity: Acetyl-CoA + choline = CoA + O-acetylcholine. Defects in CHAT are the cause of familial infantile myasthenia gravis 2 (FIMG2), also known as CMS-EA. FIMG2 patients have myasthenic symptoms since birth or early infancy, negative tests for anti-AChR antibodies, and abrupt episodic crises with increased weakness, bulbar paralysis, and apnea precipitated by undue exertion, fever, or excitement. Inheritance is autosomal recessive. Also known as: CHOACTase, choline acetylase, choline O-acetyltransferase, CMS1A, CMS1A2, B230380D24Rik.

Pathways: Skeletal Muscle Fiber Development