IHC, WB. Use at a concentration of 10-50 μg,ml. The optimal working concentration should be determined by the end user. Not yet tested in other applications.
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Reconstitute in 500 μL of sterile water. Centrifuge to remove any insoluble material.
Handling Advice
Avoid freeze and thaw cycles.
Storage
4 °C/-20 °C
Storage Comment
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Autophagocytosis is a starvation-induced process responsible for transport of cytoplasmic proteins to the vacuole. FUNCTION: Plays a role in autophagy. ATG12-conjugating enzyme (E2-like enzyme), likely serves as an ATG5-recognition molecule. SUBUNIT: Interacts with MAP1LC3A. By interacting with MAP1LC3A, it plays a role in the conjugation of ATG12 to ATG5. Also able to directly interact either with ATG5 or ATG7. SUBCELLULAR LOCATION: Cytoplasm.,Mammalian Autophagy,APG10L, APG10-like, APG10