Beclin 1 antibody

Catalog No. ABIN349986

Product Details anti-Beclin 1 Antibody

Target: Beclin 1 (BECN1) (Beclin 1, Autophagy Related (BECN1))

Reactivity: Human

Host: Sheep

Clonality: Polyclonal

Conjugate: This Beclin 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Specificity: Specific for Beclin-1.

Cross-Reactivity: Human, Marmoset, Mouse, Rat

Cross-Reactivity (Details): Other species not yet tested.

Purification: IgG

Immunogen: A synthetic peptide from human Beclin-1 conjugated to blue carrier protein has been used as the antigen. The antigen is homologous in rat and mouse.

Isotype: IgG

Application Details

Application Notes: IHC, WB. Use at a concentration of 10-50 μg,ml. The optimal working concentration should be determined by the end user. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in 500 μL of sterile water. Centrifuge to remove any insoluble material.

Handling Advice: Avoid freeze and thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date: 12 months

Target Details for Beclin 1

Target: Beclin 1 (BECN1) (Beclin 1, Autophagy Related (BECN1))

Alternative Name: Beclin-1 (BECN1 Products)

Background: Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. It may play a role in antiviral host defense. Protects against infection by a neurovirulent strain of Sindbis virus. May play a role in autophagy. SUBCELLULAR LOCATION: Cytoplasm. Golgi apparatus membrane, Peripheral membrane protein. Note: Expressed in dendrites and cell bodies of cerebellar Purkinje cells TISSUE SPECIFICITY: Ubiquitous,Mammalian Autophagy,Beclin-1, BECN1, GT197, ATG6, VPS30, beclin1

UniProt: Q14457

Pathways: Autophagy