PRNP antibody

Catalog No. ABIN349704

Product Details anti-PRNP Antibody

Target: PRNP (Prion Protein (PRNP))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PRNP antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: The mouse monoclonal antibody EM-20 recognizes human prion protein (PrP). Diglycosylated form of PrP has ~ 40 kDa, monoglycosylated form ~ 30 kDa, and nonglycosylated form ~ 19-21 kDa. This antibody is suitable for discrimination between normal cellular prion protein (PrPc) and its conformationally changed form (PrPSc) prion protein.

Cross-Reactivity (Details): Human

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: Recombinant human prion protein

Clone: EM-20

Isotype: IgG2a

Application Details

Application Notes: Western blotting: Recommended dilution: 0.5 μg/mL, non-reducing conditions are essential.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-PRNP antibody (ABIN349704)

Dvorakova, Vranac, Janouskova, ?ernilec, Koren, Lukan, Nováková, Matej, Holada, ?urin Šerbec: "Detection of the GPI-anchorless prion protein fragment PrP226* in human brain." in: BMC neurology, Vol. 13, pp. 126, (2013) (PubMed).

Target Details for PRNP

Target: PRNP (Prion Protein (PRNP))

Abstract: PRNP Products

Background: Prion protein,CD230 / human prion protein (PrP), also known as PRNP, is a ubiquitously expressed GPI-anchored cell surface glycoprotein associating with lipid raft components and functioning as a signaling molecule. CD230 / PrP plays a role in apoptosis in a cell context-dependent manner, is involved in proliferation of epithelial cells and in distribution of junction-associated proteins in human enterocytes. Conversion of this normal cellular prion protein (PrPc) into an abnormal conformer (PrPSc) is the crucial step associated with triggering the pathogenesis of the prion neurodegenerative disorders, such as the Creutzfeld-Jakob disease (CJD). Whereas PrPc is rich in alpha-helices, the PrPSc form has higher content of beta-sheets and is resistant to proteinase K.,Major prion protein, PrP, PrP27-30, PrP33-35C, ASCR, CD230, PRNP, CJD, KURU, PRIP, PRPC

Gene ID: 5621

UniProt: P04156

Pathways: Transition Metal Ion Homeostasis, Activated T Cell Proliferation