UBE2J2 antibody (Internal Region)

Catalog No. ABIN349639

Product Details anti-UBE2J2 Antibody

Target: UBE2J2 (Ubiquitin-Conjugating Enzyme E2, J2 (UBE2J2))

Binding Specificity: Internal Region

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBE2J2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Specificity: This affinity purified antibody is directed against human Ube2j2 protein. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography. A BLAST analysis was used to suggest cross-reactivity with Ube2j2 protein from human, horse, opossum, cattle, dog, mouse, rat, macaque, salmon, chicken, zebra finch, Xenopus and platypus based on 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.

Cross-Reactivity: Mouse (Murine), Rat (Rattus)

Characteristics: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Ube2j2 and Ube2j1 are homologs of the yeast ubiquitin-conjugating enzyme UBC6, which catalyzes the covalent attachment of ubiquitin to other proteins. These proteins constitute a distinct family of ubiquitin-conjugating enzymes sharing a conserved non-canonical active site sequence and a C-terminal trans-membrane domain. By analogy with yeast UBC6, Ube2j1 and Ube2j2 are localized to the endoplasmic reticulum and seem to function in the selective degradation of misfolded membrane proteins and in general mediation of the stress response.

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of the human Ube2j2 protein.
Immunogen Type: Peptide

Isotype: IgG

Application Details

Application Notes: This affinity purified antibody has been tested for use in ELISA, western blotting and immunoprecipitation. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 29 kDa in size corresponding to Ube2j2 protein by western blotting in the appropriate cell lysate or extract.

Comment:

Gene Name: UBE2J2

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.64 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store secondary antibody 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is three (3) months from date of opening.

Expiry Date: 3 months

Target Details for UBE2J2

Target: UBE2J2 (Ubiquitin-Conjugating Enzyme E2, J2 (UBE2J2))

Abstract: UBE2J2 Products

Synonyms: NCUBE-2 antibody, NCUBE2 antibody, PRO2121 antibody, 1200007B18Rik antibody, 2400008G19Rik antibody, 5730472G04Rik antibody, AL022923 antibody, Ubc6 antibody, Ubc6p antibody, zgc:162164 antibody, ubiquitin conjugating enzyme E2 J2 antibody, ubiquitin-conjugating enzyme E2J 2 antibody, ubiquitin-conjugating enzyme E2, J2 antibody, ubiquitin-conjugating enzyme E2 J2 antibody, ubiquitin-conjugating enzyme E2, J2 (UBC6 homolog, yeast) antibody, ubiquitin conjugating enzyme E2 J2 L homeolog antibody, UBE2J2 antibody, Ube2j2 antibody, LOC5574000 antibody, CpipJ_CPIJ008956 antibody, ube2j2 antibody, uce2 antibody, ube2j2.L antibody, LOC100067387 antibody

Background: This antibody is designed, produced, and is suitable for Cancer, Immunology and Nuclear Signaling research. Ube2j2 and Ube2j1 are homologs of the yeast ubiquitin-conjugating enzyme UBC6, which catalyzes the covalent attachment of ubiquitin to other proteins. These proteins constitute a distinct family of ubiquitin-conjugating enzymes sharing a conserved non-canonical active site sequence and a C-terminal trans-membrane domain. By analogy with yeast UBC6, Ube2j1 and Ube2j2 are localized to the endoplasmic reticulum and seem to function in the selective degradation of misfolded membrane proteins and in general mediation of the stress response.
Synonyms: Ubiquitin-conjugating enzyme E2 J2 Non-canonical ubiquitin-conjugating enzyme 2 Ube2j2

Gene ID: 118424, 251757431

UniProt: Q8N2K1