UBE2J2 antibody (Internal Region)

Catalog No. ABIN349639

Supplier Product No.: 600-401-b43

Product Details anti-UBE2J2 Antibody

Target: UBE2J2 (Ubiquitin-Conjugating Enzyme E2, J2 (UBE2J2))

Binding Specificity: Internal Region

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBE2J2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Supplier Product No.: 600-401-b43

Supplier: Rockland

Purpose: UBE2J2 Antibody

Cross-Reactivity (Details): This affinity purified antibody is directed against human Ube2j2 protein.

Characteristics: Synonyms: rabbit anti-UBE2J2 antibody, rabbit anti-E2 J2 antibody, rabbit anti-Ubiquitin conjugating enzyme E2J2 antibody, Non-canonical ubiquitin-conjugating enzyme 2, Ube2j2, E2 ubiquitin-conjugating enzyme J2, NCUBE-2

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Sterility: Sterile filtered

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of the human Ube2j2 protein.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Application Note: This affinity purified antibody has been tested for use in ELISA, western blotting and immunoprecipitation. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 29 kDa in size corresponding to Ube2j2 protein by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:500 - 1:3,000

Immunoprecipitation Dilution: User Optimized

ELISA Dilution: 1:5,000 - 1:25,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.64 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for UBE2J2

Target: UBE2J2 (Ubiquitin-Conjugating Enzyme E2, J2 (UBE2J2))

Alternative Name: UBE2J2 (UBE2J2 Products)

Background: Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Ube2j2 and Ube2j1 are homologs of the yeast ubiquitin-conjugating enzyme UBC6, which catalyzes the covalent attachment of ubiquitin to other proteins. These proteins constitute a distinct family of ubiquitin-conjugating enzymes sharing a conserved non-canonical active site sequence and a C-terminal trans-membrane domain. By analogy with yeast UBC6, Ube2j1 and Ube2j2 are localized to the endoplasmic reticulum and seem to function in the selective degradation of misfolded membrane proteins and in general mediation of the stress response.

Gene ID: 118424, 251757431

UniProt: Q8N2K1