LGR4 antibody (Internal Region)

Catalog No. ABIN349637

Supplier Product No.: 200-301-b45

Product Details anti-LGR4 Antibody

Target: LGR4 (Leucine-Rich Repeat Containing G Protein-Coupled Receptor 4 (LGR4))

Binding Specificity: Internal Region

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This LGR4 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Supplier Product No.: 200-301-b45

Supplier: Rockland

Purpose: LGR4 Antibody

Cross-Reactivity (Details): This antibody is specific for human LGR4 protein.

Characteristics: Synonyms: mouse anti-LGR4 antibody, mouse anti-LGR 4 antibody, leucine-rich repeat-containing G protein-coupled receptor 4

Purification: Anti-LGR4 purified from concentrated tissue culture supernate by Protein A chromatography.

Sterility: Sterile filtered

Immunogen:

Immunogen: This monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human LGR4 protein. The hybridoma was produced by fusing BALB/c mouse splenocytes and mouse myeloma SP2/O cells using conventional technology.

Immunogen Type: Conjugated Peptide

Clone: 6G8-B3-G5-C3

Isotype: IgG2b kappa

Application Details

Application Notes:

Immunohistochemistry Dilution: 5 μg/mL

Application Note: Anti-LGR4 monoclonal antibody has been tested by ELISA and western blot, and is suitable in immunohistochemistry. Expect a band approximately 102 kDa in size corresponding to LGR4 protein by western blotting in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user. Use formalin-fixed paraffin-embedded sections for immunohistochemistry. No pre-treatment of sample is required.

Western Blot Dilution: 1:500 - 1:3,000

ELISA Dilution: 1:20,000 - 1:100,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.13 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-LGR4 antibody (ABIN349637)

Yi, Xiong, Gong, Bellister, Ellis, Liu: "Analysis of LGR4 receptor distribution in human and mouse tissues." in: PLoS ONE, Vol. 8, Issue 10, pp. e78144, (2014) (PubMed).

Target Details for LGR4

Target: LGR4 (Leucine-Rich Repeat Containing G Protein-Coupled Receptor 4 (LGR4))

Alternative Name: LGR4 (LGR4 Products)

Background: Background: LGR4, also known as leucine-rich repeat-containing G protein-coupled receptor 4, is a G protein-coupled receptors (GPCRs). GPCRs are membrane bound proteins that play key roles in a variety of physiologic functions. Members of the leucine-rich GPCR (LGR) family, such as GPR48, have multiple N-terminal leucine-rich repeats (LRRs) and a 7-transmembrane domain. LGR4 is an orphan GPCR reported to be expressed in steroidogenic tissues such as placenta, ovary, testis, adrenal, pancreas, prostate, and thyroid, as well as in spinal cord, stomach, heart, and kidney.

Gene ID: 55366, 157694513

UniProt: Q8N537