CENPQ antibody

Catalog No. ABIN349636

Supplier Product No.: 200-401-b48

Product Details anti-CENPQ Antibody

Target: CENPQ (Centromere Protein Q (CENPQ))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CENPQ antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Fluorescence Microscopy (FM), Multiplex Assay (MA)

Supplier Product No.: 200-401-b48

Supplier: Rockland

Purpose: CENP-Q Antibody

Cross-Reactivity (Details): This antibody is specific for human CENP-Q protein.

Characteristics: Synonyms: rabbit anti-CENP-Q Antibody, CenpQ, centromere protein Q, CENP Q

Purification: This product was protein A purified from monospecific antiserum by immunoaffinity chromatography using protein A coupled to agarose beads.

Sterility: Sterile filtered

Immunogen:

Immunogen: This protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full-length human CENP-Q recombinant protein.

Immunogen Type: Recombinant Protein

Isotype: IgG

Application Details

Application Notes:

Application Note: This protein A purified antibody has been tested for use in ELISA, immunofluorescence microscopy and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 26-31 kDa in size corresponding to human CENP-Q by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:100 - 1:500

ELISA Dilution: 1:5,000 1:20,000

IF Microscopy Dilution: User Optimized

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.15 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for CENPQ

Target: CENPQ (Centromere Protein Q (CENPQ))

Alternative Name: CENPQ (CENPQ Products)

Background: Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Cenp-Q (also known as centromere protein Q or CENPQ) is a nuclear/centromeric protein that is one of the critical components that constitutes the CENP-O complex at the kinetochores and appears to stabilize PBIP1/CENP-U(50)/MLF1IP in the complex. This complex is important for proper recruitment of polo-like kinase 1 (Plk1) to the mitotic kinetochores. A failure in this process results in improper microtubule attachment to the kinetochores and chromosome missegregation that ultimately lead to aneuploidy.

Gene ID: 55166, 40068061

UniProt: Q7L2Z9