AKT 1/2/3 antibody

Catalog No. ABIN349616

Supplier Product No.: 200-301-401

Product Details anti-AKT 1/2/3 Antibody

Target: AKT 1/2/3

Reactivity: Human, Mouse, Rat, Chimpanzee

Host: Mouse

Clonality: Monoclonal

Conjugate: This AKT 1/2/3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), ELISA

Supplier Product No.: 200-301-401

Supplier: Rockland

Purpose: AKT Antibody

Cross-Reactivity (Details): This antibody is specific for human and mouse AKT protein.

Characteristics: Synonyms: mouse anti-AKT Antibody, RAC-PK-alpha, Protein kinase B, PKB, C-AKT, RAC-alpha serine/threonine-protein kinase, Proto-oncogene c-Akt, AKT1, AKT2, AKT3

Purification: Anti-AKT Antibody was purified by Protein A chromatography.

Sterility: Sterile filtered

Immunogen:

Immunogen: Anti-AKT Antibody was produced by repeated immunizations in mice with a synthetic peptides corresponding to residues internal to the human AKT1, AKT2, and AKT3 proteins.

Immunogen Type: Conjugated Peptide

Clone: 14E5-16C8-25F6

Isotype: IgG

Application Details

Application Notes:

Flow Cytometry Dilution: User Optimized

Immunohistochemistry Dilution: 20 μg/mL

Application Note: Mouse Anti-AKT Antibody is tested for ELISA and western blotting. This antibody is suitable for immunohistochemistry and immunoprecipitation. Expect a band approximately 54 - 56 kDa in size corresponding to AKT protein by western blotting in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user. For immunohistochemistry we recommend the use of fresh frozen tissues. Attempts at staining paraffin-embedded formalin fixed tissues were negative. No pre-treatment of sample is required.

Western Blot Dilution: 1:500 - 1:3,000

ELISA Dilution: 1:20,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-AKT 1/2/3 antibody (ABIN349616)

Figliozzi, Chen, Hsia: "New insights on thyroid hormone mediated regulation of herpesvirus infections." in: Cell & bioscience, Vol. 7, pp. 13, (2017) (PubMed).

Target Details for AKT 1/2/3

Target: AKT 1/2/3

Alternative Name: AKT1, AKT2, AKT3 (AKT 1/2/3 Products)

Background: Background: AKT is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as AKT1, Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways. AKT exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis, (ii) promotion of proliferation. Anti-AKT Antibody is ideal for investigators involved in Cell Signaling, Neuroscience, Signal Transduction research.

Gene ID: 207, 62241011

UniProt: P31749