TRIM29 antibody (acLys116)

Catalog No. ABIN349613

Supplier Product No.: 600-401-b16

Product Details anti-TRIM29 Antibody

Target: TRIM29 (Tripartite Motif Containing 29 (TRIM29))

Binding Specificity: acLys116

Reactivity: Human, Cow, Horse, Macaque, Chimpanzee

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TRIM29 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Supplier Product No.: 600-401-b16

Supplier: Rockland

Purpose: ATDC Ac-K116 Antibody

Cross-Reactivity (Details): This antibody reacts with over-expressed, acetylated lysine ATDC protein.

Characteristics: Synonyms: rabbit anti-ATDC Ac-K116 Antibody, rabbit anti-ATDC acetylated Lys116 Antibody, Ataxia-telangiectasia group D associated protein antibody, FLJ36085 antibody, TRIM 29 antibody, Tripartite motif containing protein 29 antibody, TRIM-29

Purification: This product was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Sterility: Sterile filtered

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a peptide corresponding to an internal portion of human ATDC protein around lysine 116.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Application Note: This affinity purified antibody has been tested for use in ELISA and western blotting.   Specific conditions for reactivity and detection of ATDC Ac-K116 should be optimized by the end user.  Expect a band approximately ~66 kDa in size corresponding to ATDC Ac-K116 by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:500 to 3,000

ELISA Dilution: 1:100,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.67 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-TRIM29 antibody (ABIN349613)

Yuan, Peng, Radhakrishnan, Seto: "Histone deacetylase 9 (HDAC9) regulates the functions of the ATDC (TRIM29) protein." in: The Journal of biological chemistry, Vol. 285, Issue 50, pp. 39329-38, (2011) (PubMed).

Yuan, Villagra, Peng, Coppola, Glozak, Sotomayor, Chen, Lane, Seto: "The ATDC (TRIM29) protein binds p53 and antagonizes p53-mediated functions." in: Molecular and cellular biology, Vol. 30, Issue 12, pp. 3004-15, (2010) (PubMed).

Target Details for TRIM29

Target: TRIM29 (Tripartite Motif Containing 29 (TRIM29))

Alternative Name: TRIM29 (TRIM29 Products)

Background: Background: Ataxia-telangiectasia group D-associated protein (ATDC), also called tripartite motif-containing protein 29 (TRIM29), is a novel Histone deacetylase (HDAC) associated protein. Its function is tightly regulated by HDAC. ATDC Lysine 116 (K116) is acetylated and has a significant functional role in regulating cell survival and tumorigenesis. ATDC is expressed in placenta, prostate and thymus, and is over expressed in pancreatic and cervical tumors. Its function in tumor cells is not fully understood. It is constitutively phosphorylated by PKC on serine/threonine in A431 cells. The ATDC gene product is one of a group of proteins that share multiple zinc finger motifs and an adjacent leucine zipper motif. These proteins have been proposed to form homo- or heterodimers involved in nucleic acid binding, consistent with the fact that many of these proteins appear to be transcriptional regulatory factors involved in carcinogenesis and/or differentiation. The likelihood that the ATDC gene product is involved in transcriptional regulation could explain the pleiomorphic characteristics of AT, including abnormal cell cycle regulation.

Gene ID: 23650, 17402909

UniProt: Q14134

Pathways: p53 Signaling, Apoptosis, DNA Damage Repair, Inositol Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus