TRIM29 antibody (Internal Region)

Catalog No. ABIN349613

Product Details anti-TRIM29 Antibody

Target: TRIM29 (Tripartite Motif Containing 29 (TRIM29))

Binding Specificity: Internal Region

Reactivity: Human, Cow, Horse, Macaque, Chimpanzee

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TRIM29 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Specificity: This product was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with over-expressed, acetylated lysine ATDC protein.  A BLAST analysis was used to suggest cross-reactivity with ATDC from human, horse, cattle, chimpanzee and macaque based on a 100% homology with the immunizing sequence.  Partial reactivity is expected against rat and mouse ATDC based on 92% homology with the immunizing sequence.  Cross-reactivity with ATDC from other sources has not been determined.

Cross-Reactivity: Horse (Equine), Sheep (Ovine), Chimpanzee, Macaque

Characteristics: Ataxia-telangiectasia group D-associated protein (ATDC), also called tripartite motif-containing protein 29 (TRIM29), is a novel Histone deacetylase (HDAC) associated protein. Its function is tightly regulated by HDAC. ATDC Lysine 116 (K116) is acetylated and has a significant functional role in regulating cell survival and tumorigenesis. ATDC is expressed in placenta, prostate and thymus, and is over expressed in pancreatic and cervical tumors. Its function in tumor cells is not fully understood. It is constitutively phosphorylated by PKC on serine/threonine in A431 cells. The ATDC gene product is one of a group of proteins that share multiple zinc finger motifs and an adjacent leucine zipper motif. These proteins have been proposed to form homo- or heterodimers involved in nucleic acid binding, consistent with the fact that many of these proteins appear to be transcriptional regulatory factors involved in carcinogenesis and/or differentiation. The likelihood that the ATDC gene product is involved in transcriptional regulation could explain the pleiomorphic characteristics of AT, including abnormal cell cycle regulation.

Purification: affinity purified

Sterility: Sterile filtered

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a peptide corresponding to an internal portion of human ATDC protein around lysine 116.
Immunogen Type: Peptide

Isotype: IgG

Application Details

Application Notes: This affinity purified antibody has been tested for use in ELISA and western blotting.   Specific conditions for reactivity and detection of ATDC Ac-K116 should be optimized by the end user.  Expect a band approximately ~66 kDa in size corresponding to ATDC Ac-K116 by western blotting in the appropriate cell lysate or extract.

Comment:

Gene Name: TRIM29

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.67 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is three (3) months from date of opening.

Expiry Date: 3 months

References for anti-TRIM29 antibody (ABIN349613)

Yuan, Peng, Radhakrishnan, Seto: "Histone deacetylase 9 (HDAC9) regulates the functions of the ATDC (TRIM29) protein." in: The Journal of biological chemistry, Vol. 285, Issue 50, pp. 39329-38, (2011) (PubMed).

Yuan, Villagra, Peng, Coppola, Glozak, Sotomayor, Chen, Lane, Seto: "The ATDC (TRIM29) protein binds p53 and antagonizes p53-mediated functions." in: Molecular and cellular biology, Vol. 30, Issue 12, pp. 3004-15, (2010) (PubMed).

Target Details for TRIM29

Target: TRIM29 (Tripartite Motif Containing 29 (TRIM29))

Alternative Name: ATDC Ac-K116 (TRIM29 Products)

Synonyms: ATDC antibody, 1110047J21Rik antibody, 2810431N19Rik antibody, 4732461M22Rik antibody, AI119726 antibody, MGC53484 antibody, TRIM29 antibody, atdc antibody, tripartite motif containing 29 antibody, tripartite motif-containing 29 antibody, tripartite motif containing 29 L homeolog antibody, TRIM29 antibody, Trim29 antibody, trim29.L antibody, trim29 antibody

Background: Ataxia-telangiectasia group D-associated protein (ATDC), also called tripartite motif-containing protein 29 (TRIM29), is a novel Histone deacetylase (HDAC) associated protein. Its function is tightly regulated by HDAC. ATDC Lysine 116 (K116) is acetylated and has a significant functional role in regulating cell survival and tumorigenesis. ATDC is expressed in placenta, prostate and thymus, and is over expressed in pancreatic and cervical tumors. Its function in tumor cells is not fully understood. It is constitutively phosphorylated by PKC on serine/threonine in A431 cells. The ATDC gene product is one of a group of proteins that share multiple zinc finger motifs and an adjacent leucine zipper motif. These proteins have been proposed to form homo- or heterodimers involved in nucleic acid binding, consistent with the fact that many of these proteins appear to be transcriptional regulatory factors involved in carcinogenesis and/or differentiation. The likelihood that the ATDC gene product is involved in transcriptional regulation could explain the pleiomorphic characteristics of AT, including abnormal cell cycle regulation.
Synonyms: Ataxia-telangiectasia group D associated protein antibody, ATDC antibody, FLJ36085 antibody, TRIM 29 antibody, Tripartite motif containing protein 29 antibody

Gene ID: 23650, 17402909

UniProt: Q14134

Pathways: p53 Signaling, Apoptosis, DNA Damage Repair, Inositol Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus