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DYKDDDDK Tag antibody

Rockland 200-301-383 WB, ELISA, IHC, FACS Host: Mouse Monoclonal 29E4-G7 unconjugated
Rockland
Catalog No. ABIN349610
Supplier Product No.: 200-301-383
  • Target See all DYKDDDDK Tag products
    DYKDDDDK Tag
    Reactivity
    Please inquire
    Host
    • 52
    • 48
    • 8
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    • 6
    Mouse
    Clonality
    • 61
    • 59
    Monoclonal
    Conjugate
    • 40
    • 15
    • 10
    • 5
    • 3
    • 3
    • 2
    • 2
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    • 2
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    • 2
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    • 1
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    • 1
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    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
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    • 1
    This DYKDDDDK Tag antibody is un-conjugated
    Application
    • 79
    • 62
    • 27
    • 21
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    • 8
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    • 1
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    Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)
    Supplier Product No.
    200-301-383
    Supplier
    Rockland
    Purpose
    DYKDDDDK Tag (Anti-FLAG®) Antibody
    Sequence
    DYKDDDDK
    Cross-Reactivity (Details)
    The purified antibody is directed against the FLAG™ motif and is useful in determining its presence in various assays where the epitope tag is present at either the amino or carboxy terminus of recombinant proteins.
    Characteristics
    Synonyms: mouse anti-FLAG™ tag, Enterokinase Cleavage Site (ECS), mouse anti-DYKDDDDK, Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys
    Purification
    This product is an IgG fraction antibody purified from ascites by Protein A chromatography followed by extensive dialysis against the buffer stated above.
    Sterility
    Sterile filtered
    Immunogen

    Immunogen: This antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to the FLAG™ epitope tag peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH using maleimide.

    Immunogen Type: Conjugated Peptide

    Clone
    29E4-G7
    Isotype
    IgG2a kappa
  • Application Notes

    Flow Cytometry Dilution: User Optimized

    Immunohistochemistry Dilution: User Optimized

    Application Note: Anti-FLAG has been tested by ELISA and western blot. Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein's biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells. Rockland Immunochemicals produces anti-epitope tag antibodies against many common epitope tags including Myc, GST, GFP, 6X His, MBP, FLAG™ and HA. Rockland Immunochemicals also produces antibodies to other tags including FITC, Rhodamine (TRITC), DNP and biotin.

    Western Blot Dilution: 1:2,000 - 1:20,000

    ELISA Dilution: 1:150,000 - 1:250,000

    Other: User Optimized

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1.0 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    Expiry Date
    12 months
  • Turchinovich, Surowy, Tonevitsky, Burwinkel: "Interference in transcription of overexpressed genes by promoter-proximal downstream sequences." in: Scientific reports, Vol. 6, pp. 30735, (2018) (PubMed).

    Saha, Parks: "Human adenovirus type 5 vectors deleted of early region 1 (E1) undergo limited expression of early replicative E2 proteins and DNA replication in non-permissive cells." in: PLoS ONE, Vol. 12, Issue 7, pp. e0181012, (2017) (PubMed).

    Wu, Kim, Seravalli, Barycki, Hart, Gohara, Di Cera, Jung, Kosman, Lee: "Potassium and the K+/H+ Exchanger Kha1p Promote Binding of Copper to ApoFet3p Multi-copper Ferroxidase." in: The Journal of biological chemistry, Vol. 291, Issue 18, pp. 9796-806, (2016) (PubMed).

    Lee, Seo, Back, Han, Jeong, Lee, Choi, Han: "Transcriptional regulation of Niemann-Pick C1-like 1 gene by liver receptor homolog-1." in: BMB reports, Vol. 48, Issue 9, pp. 513-8, (2016) (PubMed).

  • Target
    DYKDDDDK Tag
    Alternative Name
    FLAG (DYKDDDDK Tag Products)
    Target Type
    Tag
    Background
    Background: Antibody for the detection of FLAG™ recognizes FLAG™ and is optimally suited for monitoring the expression of FLAG™ tagged fusion proteins. Antibody for the detection of FLAG™ can be used to identify fusion proteins containing the FLAG™ epitope. Antibody for the detection of FLAG™ recognizes the epitope tag fused to either the amino- or carboxy- termini of targeted proteins. The epitope tag peptide sequence was first derived from the 11-amino-acid leader peptide of the gene-10 product from bacteriophage T7. DYKDDDDK is the most commonly used hydrophilic octapeptide tag.
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