GFP antibody

Catalog No. ABIN349609

Product Details anti-GFP Antibody

Target: GFP (Green Fluorescent Protein (GFP))

Reactivity: Aequorea victoria

Host: Chicken

Clonality: Polyclonal

Conjugate: This GFP antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Fluorescence Microscopy (FM), Dot Blot (DB)

Purpose: GFP Antibody

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Chicken Serum and purified and partially purified Green Fluorescent Protein (Aequorea victoria).

Characteristics: Synonyms: chicken anti-GFP antibody, Green Fluorescent Protein, GFP antibody, Green Fluorescent Protein antibody, EGFP, enhanced Green Fluorescent Protein, Aequorea victoria, Jellyfish

Purification: Anti-GFP IgY was prepared from egg yolks by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Sterility: Sterile filtered

Immunogen:

Immunogen: The immunogen is a Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.

Immunogen Type: Recombinant Protein

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:200 - 1:1,000

Application Note: Polyclonal anti-GFP is designed to detect GFP and its variants. Anti-GFP Antibody has been tested by ELISA, dot blot, and western and is suitable in immunofluorescence. This antibody can be used to detect GFP by ELISA (sandwich or capture) for the direct binding of antigen and recognizes wild type, recombinant and enhanced forms of GFP. Biotin conjugated polyclonal anti-GFP used in a sandwich ELISA is well suited to titrate GFP in solution using this antibody in combination with Rockland's monoclonal anti-GFP (600-301-215) using either form of the antibody as the capture or detection antibodies. However, use the monoclonal form only for the detection of wild type or recombinant GFP as this form does not sufficiently detect 'enhanced' GFP. The detection antibody is typically conjugated to biotin and subsequently reacted with streptavidin conjugated HRP (code # S000-03). Fluorochrome conjugated polyclonal anti-GFP can be used to detect GFP by immunofluorescence microscopy in prokaryotic (E.coli) and eukaryotic (CHO cells) expression systems and can detect GFP containing inserts. Significant amplification of signal is achieved using fluorochrome conjugated polyclonal anti-GFP relative to the fluorescence of GFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated polyclonal anti-GFP to detect GFP or GFP containing proteins on western blots.

Western Blot Dilution: 1:1,000 - 1:5,000

ELISA Dilution: 1:10,000 - 1:80,000

IF Microscopy Dilution: User Optimized

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.64 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store GFP Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-GFP antibody (ABIN349609)

Li, Cao, Xiao, Tang, Deng, Yang, Yoshii, Luo: "Hub-organized parallel circuits of central circadian pacemaker neurons for visual photoentrainment in Drosophila." in: Nature communications, Vol. 9, Issue 1, pp. 4247, (2019) (PubMed).

Goswami, Wang, Zhang, Xiao, Karlen, Li, Zawadzki, Burns, Lam, Pugh: "Novel window for cancer nanotheranostics: non-invasive ocular assessments of tumor growth and nanotherapeutic treatment efficacy in vivo." in: Biomedical optics express, Vol. 10, Issue 1, pp. 151-166, (2019) (PubMed).

Chiu, Martenson, Yamazaki, Natsume, Sakimura, Tomita, Tavalin, Higley: "Input-Specific NMDAR-Dependent Potentiation of Dendritic GABAergic Inhibition." in: Neuron, Vol. 97, Issue 2, pp. 368-377.e3, (2018) (PubMed).

Maruoka, Kedashiro, Ueda, Mizutani, Takai: "Nectin-4 co-stimulates the prolactin receptor by interacting with SOCS1 and inhibiting its activity on the JAK2-STAT5a signaling pathway." in: The Journal of biological chemistry, Vol. 292, Issue 17, pp. 6895-6909, (2017) (PubMed).

Boye, Alexander, Witherspoon, Boye, Peterson, Clark, Sandefer, Girkin, Hauswirth, Gamlin: "Highly Efficient Delivery of Adeno-Associated Viral Vectors to the Primate Retina." in: Human gene therapy, Vol. 27, Issue 8, pp. 580-97, (2017) (PubMed).

Kim, Fenk, Lyu, Maimon: "Quantitative Predictions Orchestrate Visual Signaling in Drosophila." in: Cell, Vol. 168, Issue 1-2, pp. 280-294.e12, (2017) (PubMed).

Chen, Luhur, Sokol: "Lin-28 promotes symmetric stem cell division and drives adaptive growth in the adult Drosophila intestine." in: Development (Cambridge, England), Vol. 142, Issue 20, pp. 3478-87, (2016) (PubMed).

He: "miRNA Tagging and Affinity-purification (miRAP)." in: Bio-protocol, Vol. 2, Issue 19, (2012) (PubMed).

Tseng, Firestein: "The role of PSD-95 and cypin in morphological changes in dendrites following sublethal NMDA exposure." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 31, Issue 43, pp. 15468-80, (2011) (PubMed).

Target Details for GFP

Target: GFP (Green Fluorescent Protein (GFP))

Alternative Name: GFP (GFP Products)

Background: Background: Anti-GFP antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. GFP antibody is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Chicken IgY lacks the classic "Fc" domain and does not bind to mammalian IgG Fc receptors. Thus resulting in lower Backgrounds for western blotting, ELISA and Immunohistochemistry.