LacI antibody

Catalog No. ABIN349605

Product Details

Target: LacI

Reactivity: E. coli, Shigella sonnei

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Specificity: This product was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with recombinant Lac I protein. A BLAST analysis was used to suggest cross-reactivity with Lac I from DNA-binding transcriptional repressor in Escherichia coli (strain DH10B) and transcriptional repressor of the lac operon in Shigella sonnei (strain Ss046) based on a 100% homology with the immunizing sequence. Cross-reactivity with Lac I from other sources has not been determined.

Cross-Reactivity: Shigella Sonnei (S. sonnei)

Characteristics: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. The lac repressor protein (Lac I) is an important genetic control protein. It controls the expression of the lactose metabolizing enzymes in Escherichia coli. This regulation involves specific recognition of the operator DNA sequence (Lac O) and consequent inhibition of transcription initiation by RNA polymerase, modulation of binding at this target site by small sugar molecules, inducers, provides the basis for regulation. Different ligand binding activities are associated with the isolated domains of Lac I: specific and nonspecific DNA binding with the NH2 termini, and inducer and specific DNA binding with the core protein. This antibody could possibly be used to recover and purify protein/nucleic acid complexes (host and viral proteins associated with the viral DNA) from infected cells.

Purification: affinity purified

Sterility: Sterile filtered

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a full-length recombinant Lac I protein.
Immunogen Type: RecombinantProtein

Isotype: IgG

Application Details

Application Notes: This affinity purified antibody has been tested for use in ELISA and western blotting using recombinant Lac I protein.   Specific conditions for reactivity and detection of Lac I should be optimized by the end user.  Expect a band approximately ~38 kDa in size corresponding to Lac I by western blotting in the appropriate cell lysate or extract.

Comment:

Gene Name: LAC1

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 3 months

References

Chang, Thrall, Laureti, Piatt, Pagès, Loparo: "Compartmentalization of the replication fork by single-stranded DNA-binding protein regulates translesion synthesis." in: Nature structural & molecular biology, Vol. 29, Issue 9, pp. 932-941, (2022) (PubMed).

Kuhn, Pascual-Garcia, Gozalo, Little, Capelson: "Chromatin targeting of nuclear pore proteins induces chromatin decondensation." in: The Journal of cell biology, Vol. 218, Issue 9, pp. 2945-2961, (2020) (PubMed).

Wyatt, Xiao, Americo, Earl, Moss: "Novel Nonreplicating Vaccinia Virus Vector Enhances Expression of Heterologous Genes and Suppresses Synthesis of Endogenous Viral Proteins." in: mBio, Vol. 8, Issue 3, (2018) (PubMed).

Agelopoulos, McKay, Mann: "Developmental regulation of chromatin conformation by Hox proteins in Drosophila." in: Cell reports, Vol. 1, Issue 4, pp. 350-9, (2013) (PubMed).

Target Details

Target: LacI

Alternative Name: Lac I

Background: This antibody is designed, produced, and is suitable for Cancer, Immunology and Nuclear Signaling research. The lac repressor protein (Lac I) is an important genetic control protein. It controls the expression of the lactose metabolizing enzymes in Escherichia coli. This regulation involves specific recognition of the operator DNA sequence (Lac O) and consequent inhibition of transcription initiation by RNA polymerase; modulation of binding at this target site by small sugar molecules, inducers, provides the basis for regulation. Different ligand binding activities are associated with the isolated domains of Lac I: specific and nonspecific DNA binding with the NH2 termini, and inducer and specific DNA binding with the core protein. This antibody could possibly be used to recover and purify protein/nucleic acid complexes (host and viral proteins associated with the viral DNA) from infected cells.
Synonyms: DNA binding transcriptional repressor antibody, LacI antibody, Transcriptional repressor of the lac operon antibody

Gene ID: 8156885, 146576

UniProt: P03023