THRB antibody (N-Term)

Catalog No. ABIN349600

Supplier Product No.: 600-401-a96

Product Details anti-THRB Antibody

Target: THRB (Thyroid Hormone Receptor, beta (THRB))

Binding Specificity: N-Term

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This THRB antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP), Fluorescence Microscopy (FM)

Supplier Product No.: 600-401-a96

Supplier: Rockland

Purpose: Thyroid Hormone Receptor beta 1 Antibody

Cross-Reactivity (Details): This antibody reacts with human THRB protein.

Characteristics: Synonyms: Rabbit anti-THRB antibody, rabbit anti-Thyroid hormone receptor beta 1 antibody, Anti-THRB1 antibody, c-erbA-beta, THR1 antibody, c-erbA-2, Nuclear receptor subfamily 1 group A member 2, ERBA2, NR1A2

Purification: This product was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Sterility: Sterile filtered

Immunogen:

Immunogen: Anti-TR ß1 antibody is affinity purified from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the N-terminal of human THRB isoform 1 protein.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Application Note: This affinity purified antibody has been tested for use in ELISA, IF, and western blotting.

Western Blot Dilution: 1:500 - 1:2,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:20,000 - 1:200,000

IF Microscopy Dilution: 1:1000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store Anti-TR beta1 antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-THRB antibody (ABIN349600)

Fozzatti, Park, Zhao, Willingham, Cheng: "Oncogenic Actions of the Nuclear Receptor Corepressor (NCOR1) in a Mouse Model of Thyroid Cancer." in: PLoS ONE, Vol. 8, Issue 6, pp. e67954, (2017) (PubMed).

Zhao, Zhu, Won Park, Fozzatti, Willingham, Cheng: "Role of TSH in the spontaneous development of asymmetrical thyroid carcinoma in mice with a targeted mutation in a single allele of the thyroid hormone-β receptor." in: Endocrinology, Vol. 153, Issue 10, pp. 5090-100, (2012) (PubMed).

Mascanfroni, Montesinos, Alamino, Susperreguy, Nicola, Ilarregui, Masini-Repiso, Rabinovich, Pellizas: "Nuclear factor (NF)-kappaB-dependent thyroid hormone receptor beta1 expression controls dendritic cell function via Akt signaling." in: The Journal of biological chemistry, Vol. 285, Issue 13, pp. 9569-82, (2010) (PubMed).

Target Details for THRB

Target: THRB (Thyroid Hormone Receptor, beta (THRB))

Alternative Name: THRB (THRB Products)

Background: Background: Anti-Thyroid Hormone Receptor ß1 (TRB1) antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. The protein encoded by this gene is a nuclear hormone receptor for triiodothyronine. It is one of the several receptors for thyroid hormone, and has been shown to mediate the biological activities of thyroid hormone. Knockout studies in mice suggest that the different receptors, while having certain extent of redundancy, may mediate different functions of thyroid hormone. Defects in this gene are known to be a cause of generalized thyroid hormone resistance (GTHR), a syndrome characterized by goiter and high levels of circulating thyroid hormone (T3-T4), with normal or slightly elevated thyroid stimulating hormone (TSH). This TRB1 antibody is ideal for Immunology, Thyroid and Signal Transduction research.

Gene ID: 7068, 40806162

UniProt: P10828

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Sensory Perception of Sound