POGZ antibody (N-Term)

Catalog No. ABIN349593

Supplier Product No.: 600-401-a89

Product Details anti-POGZ Antibody

Target: POGZ (Pogo Transposable Element with ZNF Domain (POGZ))

Binding Specificity: N-Term

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POGZ antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Supplier Product No.: 600-401-a89

Supplier: Rockland

Purpose: Pogz Antibody

Cross-Reactivity (Details): This antibody is known to react with mouse Pogz protein.

Characteristics: Synonyms: rabbit anti-POGZ antibody, pogo transposable element with ZNF domain antibody, Suppressor of hairy wing homolog 5, Zinc finger protein 280E, Zinc finger protein 635, SUHW5, ZNF280E, ZNF635

Purification: This product was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Sterility: Sterile filtered

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the N-terminus of mouse Pogz protein.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Application Note: This affinity purified antibody has been tested for use in ELISA and western blotting.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 155 kDa in size corresponding to Pogz by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:2,000 to 1:20,000

ELISA Dilution: 1:100,000 - 1:400,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.6 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for POGZ

Target: POGZ (Pogo Transposable Element with ZNF Domain (POGZ))

Alternative Name: Pogz (POGZ Products)

Background: Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Pogz is a zinc-finger protein containing at least 8 C2H2 zinc fingers, a CENP-B (centromere protein-B) domain, and a DDE domain found in the bacterial transposase/retroviral integrase family. Members of this family of integrases share structural homologies and show similar catalytic activity to the RAG1 and RAG2 proteins that initiate V(D)J recombination in lymphoid progenitors.  Mouse Pogz has a human homolog with a base pair similarity of 90 % and amino acid similarity of 93 % for the full length protein.  In humans at least 3 variants have been predicted and they are all similar at the N-terminus.   A number of expressed sequence tags (ESTs) cloned from murine undifferentiated ES cells and Lin-/c-Kit+/Sca-1+ hematopoietic stem cell cDNA libraries correspond to the Pogz gene, further underlining an important function for this gene in stem cells.  In mice, deletion of the gene in all tissues early in embryogenesis has been shown to be lethal.

Gene ID: 229584

UniProt: Q8BZH4