MLF1IP antibody (N-Term)

Catalog No. ABIN349588

Product Details anti-MLF1IP Antibody

Target: MLF1IP (MLF1 Interacting Protein (MLF1IP))

Binding Specificity: N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MLF1IP antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Purpose: MLF1 Antibody

Cross-Reactivity (Details): This affinity purified antibody is directed against human MLF1IP protein.

Characteristics: Synonyms: rabbit anti-MLF1 antibody, rabbit anti-MLF1 Interacting Protein antibody, Centromere protein U, CENP-U, Centromere protein of 50 kDa, CENP-50, Interphase centromere complex protein 24, KSHV latent nuclear antigen-interacting protein 1, MLF1-interacting protein, Polo-box-interacting protein 1, ICEN24, PBIP1, KLIP1

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Sterility: Sterile filtered

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a 200 residue recombinant protein corresponding to the amino terminal end of human MLF1IP protein.

Immunogen Type: Recombinant Protein

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: User Optimized

Application Note: This affinity purified antibody has been tested for use in ELISA, Immunohistochemistry, and western blotting.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 65 kDa in size corresponding to MLF1IP protein by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:500- 1:2,000

ELISA Dilution: 1:1,400,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.88 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for MLF1IP

Target: MLF1IP (MLF1 Interacting Protein (MLF1IP))

Alternative Name: CENPU (MLF1IP Products)

Background: Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Myeloid leukemia factor-1 (MLF1) Interacting Protein (also known as PBIP1, MLF1IP1, KLIP1 or KSHV latent nuclear antigen interacting protein 1) is a novel polo-like kinase 1 (Plk1) substrate.  Plk1 phosphorylation of MLF1IP induces ubiquitination and degradation of MLF1IP prior to the metaphase/anaphase transition.  Several Plk1-dependent phosphorylation sites have been identified on MLF1IP by mass spectrometry.  Mutations of these sites stabilize MLF1IP and inhibit mitotic progression.  Subsequent in vitro and in vivo MLF1IP phosphorylation and stability assays have revealed that phosphorylation of Thr78 is critical for triggering Plk1-dependent MLF1IP degradation.  Expression of a non-degradable Thr78Ala mutant was sufficient to induce a mitotic block.  Timely phosphorylation of MLF1IP on Thr78 by Plk1 is critical for eliminating the MLF1IP-imposed mitotic block prior to anaphase onset.  MLF1IP is speculated to be a novel tumor suppressor, whose function is required for proper sister-chromatid separation.  Loss of MLF1IP function may result in improper segregation of chromosomes and genomic instability, thus promoting tumorigenesis.

Gene ID: 79682, 38016935

UniProt: Q71F23