COL6 antibody
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- Target See all COL6 Antibodies
- COL6 (Collagen, Type VI (COL6))
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Reactivity
- Human, Rabbit
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This COL6 antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro))
- Specificity
- Recognizes human type VI collagen. Does not react with denatured or reduced type VI collagen, although this antibody does recognize the triple helix consisting of alpha1 (VI), alpha 2 (VI) and alpha3 (gamma) The epitope has not been mapped and is not known to react with collagens I, II, III, IV, or V in native assays. Species cross-reactivity: rabbit.
- Purification
- Purified
- Immunogen
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Collagen Type VI.
Type of Immunogen: Purified protein - Isotype
- IgG1 kappa
- Top Product
- Discover our top product COL6 Primary Antibody
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- Application Notes
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Approved: ELISA, IHC, IHC-Fr (1 - 10 μg/mL), WB (10 μg/mL)
Usage: Please note that this antibody does not react with denatured or reduced type VI collagen. Suitable for use in ELISA, Western Blot and Immunohistochemistry. Western Blot: 10 μg/mL. Immunohistochemistry: 1-10 μg/mL on acetone fixed paraffin-embedded tissues or frozen tissues. Not recommended for formalin fixed tissues. Epitope retrieval suggestions are treatment with 0.04 % trypsin in 0.01 M CaCl2, 0.05 M Tris-HCl, pH 7.6, 37?C for 10 min before the non specific peroxidase block. - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- PBS, pH 7.0, 2 % BSA (protease-free).
- Handling Advice
- Avoid freeze-thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- Target
- COL6 (Collagen, Type VI (COL6))
- Alternative Name
- Collagen VI (COL6 Products)
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