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EGFR antibody (Tyr1173)

Name: EGFR antibody (Tyr1173)
Brand: antibodies-online
SKU: ABIN343717
Price: 406.44 USD
Availability: InStock

EGFR Reactivity: Human WB, FACS, IP Host: Mouse Monoclonal EM-13 unconjugated

Western blotting analysis of EGFR (phospho-Tyr1173) by mouse monoclonal antibody EM-13 inEGF-treated A431 (A), CALU-3 (B), MCF-7 (C), Jurkat (D) and Ramos (E) cell lines (reduced conditions). (EGFR antibody (Tyr1173))

Separation of EGF stimulated A431 cell suspension stained using anti-human EGFR (pY1173) (EM-13) purified antibody (concentration in sample 3 μg/mL, GAM PE, red-filled) from EGF stimulated A431 cell suspension unstained by primary antibody (GAM PE, black-dashed) in flow cytometry analysis (intracellular staining). (EGFR antibody (Tyr1173))

FACS

Immunoprecipitation ofEGFR from EGF-treated A431 cells by monoclonal antibodies EM-12 (A), (B), a commercial anti-EGFR polyclonal antibody (C)and anti-EGFR monoclonal mAb108 (D). (EGFR antibody (Tyr1173))

Immunoprecipitation ofEGFR from EGF-treated A431 cells byphosphospecific monoclonal antibodies EM-12 (A), (B), a commercial anti-EGFR polyclonal antibody(C) and anti-EGFR monoclonal mAb108 (D). (EGFR antibody (Tyr1173))

4 images

Catalog No. ABIN343717

Datasheet as PDF

Target See all EGFR Antibodies

EGFR (Epidermal Growth Factor Receptor (EGFR))
Binding Specificity
39

38

31

31

30

27

27

26

24

22

19

18

16

16

16

15

15

15

11

11

10

9

9

9

8

8

7

6

5

5

5

4

4

4

4

4

4

4

4

3

3

3

3

3

3

3

3

3

3

3
Tyr1173
Reactivity
744

296

283

25

25

24

19

14

8

3

2

2

1

1

1

1

1

1
Human
Host
579

166

14

13

5

1

1
Mouse
Compatible Secondaries
Clonality
541

233

2
Monoclonal
Conjugate
406

58

29

22

19

17

16

16

16

16

16

11

10

10

10

10

10

10

10

10

10

8

8

5

4

4

4

4

4

1

1

1

1

1

1
This EGFR antibody is un-conjugated
Application
532

243

147

142

127

102

99

93

82

46

37

34

34

18

11

8

6

4

4

3

3

3

3

3

3

2

2

2

2

1

1

1

1

1

1

1

1

1
Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP)

Specificity

The mouse monoclonal antibody EM-13 reacts with human EGFR (ErbB1 / HER1) phosphorylated on intracellular tyrosine 1173.

Cross-Reactivity (Details)

Human

Purification

Purified by protein-A affinity chromatography.

Purity

> 95 % (by SDS-PAGE)

Immunogen

Synthetic phospho-peptide covering sequence around tyrosine 1173 of human EGFR.

Clone

EM-13

Isotype

IgG1

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Application Notes

Western blotting: Recommended dilution: 1 μg/mL, positive control: EGF-stimulated A431, negative control: non-stimulated A431.
Flow cytometry: Recommended dilution: 1-4 μg/mL. Intracellular staining.

Restrictions

For Research Use only
Concentration

1 mg/mL

Buffer

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice

Do not freeze.

Storage

4 °C

Storage Comment

Store at 2-8°C. Do not freeze.
Target

EGFR (Epidermal Growth Factor Receptor (EGFR))

Alternative Name

EGFR (EGFR Products)

Background

Epidermal growth factor receptor,The oncoprotein EGFR (epidermal growth factor receptor), also known as HER1 / ErbB1, is a member of ErbB family of cell surface receptor tyrosine kinases. This 170 kDa transmembrane glycoprotein is often associated with cancerogenesis, although its activation state is controlled at various levels including trafficking and degradation steps. Binding of receptor-specific ligands to the EGFR ectodomain results in formation of homodimeric or heterodimeric kinase-active complexes into which HER2 / ErbB2 is recruited as a preferred partner. Subsequent signaling cascades such as RAS/MAPK and PI3K/AKT pathways lead to cell proliferation and survival responses.,EGFR, EGF-R, Her1, ERBB1, PIG61, NISBD2

Gene ID

1956

UniProt

P00533

Pathways

NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation, S100 Proteins