LST1 antibody

Catalog No. ABIN343705

Product Details anti-LST1 Antibody

Target: LST1 (Leukocyte Specific Transcript 1 (LST1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This LST1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Specificity: The antibody LST1/02 reacts with an extracellular epitope of LST1, an approximately 6-11 kDa protein expressed as various transmembrane or soluble forms. LST1 is found predominantly on monocytes and dendritic cells. It migrates on SDS PAGE gels as approximately 25-28 kDa molecule.

Cross-Reactivity (Details): Human, Other not determined

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: KLH-fused peptide corresponding to amino acids SSEGPDLRGRDKRGT of human LST1

Clone: LST1-02

Isotype: IgG1

Application Details

Application Notes: Immunoprecipitation: Positive control: RAJI human Burkitt lymphoma cell line.
Western blotting: The antigen migrates on SDS PAGE gels as approximately 25-28 kDa molecule.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

Target Details for LST1

Target: LST1 (Leukocyte Specific Transcript 1 (LST1))

Alternative Name: LST1 (LST1 Products)

Synonyms: B144 antibody, D6S49E antibody, LST-1 antibody, leukocyte specific transcript 1 antibody, LST1 antibody, Lst1 antibody

Background: Leukocyte specific transcript 1,LST1 (leukocyte-specific transcript 1, also known as B144) is expressed in cells of myeloid/erythroid lineage (monocytes, granulocytes, dendritic cells, platelets, erythrocytes). At least 14 alternatively spliced variants (LST1/A –, LST1/N) can be detected, some of them (LST1/A, B, C, G, I, K) are transmembrane cell surface-exposed forms, the other are soluble. LST1 induces production of long, thin filopodia in dendritic cells, has an inhibitory effect on lymphocyte proliferation and may have an immunomodulatory role. LST1/A is an 11 kDa transmembrane adaptor present in membrane rafts and forms spontaneously covalent homodimers. Its intracellular domain contains two tyrosine motifs, one of them being an ITIM very similar to such motifs in Siglec.,LST-1, B144

Gene ID: 7940

UniProt: O00453