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Histone 3 antibody (H3K36me3)

Name: Histone 3 antibody (H3K36me3)
SKU: ABIN3434046
Price: 666.15 USD
Availability: InStock

H3 Reactivity: Human, Mouse WB, IF, ChIP, ICC, DB, ChIP-seq, CUT&RUN Host: Rabbit Polyclonal unconjugated

Western Blot: Nuclear extract of HeLa cells (20 µg) probed with the Histone H3 trimethyl Lys36 antibody at a dilution of 1 µg/ml. (Histone 3 antibody (H3K36me3))

ChIP

ChIP-seq

Histone H3K36me3 antibody (pAb) tested by immunofluorescence. (Histone 3 antibody (H3K36me3))

Specificity Data: Dot blot analysis was used to confirm the specificity of Histone H3 trimethyl Lys36 antibody for trimethyl-lysine 36 of histone H3. (Histone 3 antibody (H3K36me3))

5 images

(5 references)

Catalog No. ABIN3434046

Datasheet as PDF

Target See all Histone 3 (H3) Antibodies

Histone 3 (H3)
Binding Specificity
57

49

47

42

40

40

36

36

35

34

34

33

32

30

28

27

26

23

23

23

22

21

20

18

18

17

16

16

16

15

15

15

15

14

13

13

13

12

12

12

11

11

10

10

10

10

9

9

8

8
H3K36me3
Reactivity
1385

956

877

59

42

41

41

40

39

35

35

30

26

22

20

6

3

3

3

3

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Human, Mouse
Host
1203

239

8

5
Rabbit
Compatible Secondaries
Clonality
1127

327

1
Polyclonal
Conjugate
796

80

48

48

48

48

48

48

34

33

21

18

18

18

17

17

17

17

17

17

17

17

12

1
This Histone 3 antibody is un-conjugated
Application
1034

407

374

315

241

188

173

166

163

153

138

127

117

48

38

34

32

22

15

14

7

7

6

4

3

1

1

1
Western Blotting (WB), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Dot Blot (DB), ChIP DNA-Sequencing (ChIP-seq), Cleavage Under Targets and Release Using Nuclease (CUT&RUN)

Purification

Protein A Chromatography

Immunogen

This Histone H3 trimethylLys36 antibody was raised against a peptide containing trimethylLys36 of human histone H3.

Isotype

IgG

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H3 Reactivity: Human, Saccharomyces cerevisiae WB, IF, ChIP, ICC, DB, ChIP-seq, CUT&RUN, CUT&Tag Host: Rabbit Polyclonal unconjugated

anti-Histone 3 (H3) (3meLys4) antibody
H3 Reactivity: Human, Saccharomyces cerevisiae WB, IF, ChIP, ICC, DB, ChIP-seq, CUT&RUN Host: Rabbit Polyclonal unconjugated

anti-Histone 3 (H3) (H3K36me3) antibody
H3 Reactivity: Human, Mouse WB, ChIP, DB, ChIP-seq, CUT&RUN, CUT&Tag Host: Mouse Monoclonal MABI 0333 unconjugated

anti-Histone 3 (H3) (H3K9ac) antibody
H3 Reactivity: Human, Mouse WB, IF, ChIP, ICC, DB, ChIP-seq, CUT&RUN, CUT&Tag Host: Rabbit Polyclonal unconjugated

anti-Histone 3 (H3) (AA 71-136) antibody
H3 Reactivity: Human, Mouse, Rat WB, ELISA, ICC, FACS, IHC (p), IF (cc), IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

anti-Histone 3 (H3) (H3K27me) antibody
H3 Reactivity: Human WB, IHC, IF, ChIP, IP, ChIP-seq Host: Rabbit Polyclonal unconjugated

anti-Histone 3 (H3) (H3K4me3) antibody
H3 Reactivity: Human, Mouse, Saccharomyces cerevisiae WB, IF, ChIP, ICC, DB, ChIP-seq, CUT&RUN, CUT&Tag Host: Rabbit Polyclonal unconjugated

anti-Histone 3 (H3) (C-Term) antibody
H3 Reactivity: Human WB, IHC, ChIP, IP Host: Rabbit Polyclonal unconjugated

anti-Histone 3 (H3) (H3K4me3) antibody
H3 Reactivity: Human WB, IHC, IF, ChIP, IP, DB, ChIP-seq, CUT&RUN, CUT&Tag Host: Rabbit Polyclonal unconjugated

Application Notes

Optimal working dilution should be determined by the investigator.

Restrictions

For Research Use only
Concentration

1 μg/μL

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice

Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Storage

-20 °C

Storage Comment

Antibodies in solution can be stored at -20 °C for 2 years.

Expiry Date

6 months
Powers, Parvanov, Baker, Walker, Petkov, Paigen: "The Meiotic Recombination Activator PRDM9 Trimethylates Both H3K36 and H3K4 at Recombination Hotspots In Vivo." in: PLoS genetics, Vol. 12, Issue 6, pp. e1006146, (2016) (PubMed).

Ho, Nateras, Yan, Park, Jensen, Borges, Lee, Champion, Tibes, Bryce, Carballido, Todd, Joseph, Wong, Parker, Stanton, Castle: "A Multidisciplinary Biospecimen Bank of Renal Cell Carcinomas Compatible with Discovery Platforms at Mayo Clinic, Scottsdale, Arizona." in: PLoS ONE, Vol. 10, Issue 7, pp. e0132831, (2015) (PubMed).

Pham, Inoue, Vu, Nguyen, Nakayashiki, Ikeda, Nakayashiki: "MoSET1 (Histone H3K4 Methyltransferase in Magnaporthe oryzae) Regulates Global Gene Expression during Infection-Related Morphogenesis." in: PLoS genetics, Vol. 11, Issue 7, pp. e1005385, (2015) (PubMed).

Feng, Zhang, Shea, Creighton, Coarfa, Hilsenbeck, Lanz, He, Wang, Fu, Nardone, Song, Bradner, Mitsiades, Mitsiades, Osborne, Schiff, OMalley: "An epigenomic approach to therapy for tamoxifen-resistant breast cancer." in: Cell research, Vol. 24, Issue 7, pp. 809-19, (2014) (PubMed).

Mews, Zee, Liu, Donahue, Garcia, Berger: "Histone methylation has dynamics distinct from those of histone acetylation in cell cycle reentry from quiescence." in: Molecular and cellular biology, Vol. 34, Issue 21, pp. 3968-80, (2014) (PubMed).
Target

Histone 3 (H3)

Alternative Name

Histone H3 (H3 Products)

Synonyms

H-3 antibody, histocompatibility 3 antibody, H3 antibody

Molecular Weight

17 kDa

Gene ID

3020