CD14 antibody
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- Target See all CD14 Antibodies
- CD14
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Reactivity
- Mouse
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This CD14 antibody is un-conjugated
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Application
- ELISA
- Specificity
- Membrane bound and soluble mouse CD14. The antibody blocks LPS binding to membrane bound CD14.
- Purification
- Protein A affinity column
- Immunogen
- Monocytes of mouse
- Clone
- BiG53
- Isotype
- IgG1
- Top Product
- Discover our top product CD14 Primary Antibody
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- Application Notes
- Suitable for immunostaining of CD14 positive cells and inhibition studies Binds at LPS activated mouse monocytes as well as mouse CD14 transfected cells or recombinant mouse CD14 ELISA. Inhibition of LPS binding to CD14 (Binding at LPS binding site). Up to 1 ug/ml biG53 inhibit binding of FITC-LPS (0.5ug/ml) to 6 x 105 CD14+CHO transfectants (FACS), Binding titre at mouse CD14 transfected CHO-cells: more than 20.000 (ELISA, Flow cytometry)
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Reconstitute with bidistilled water
- Concentration
- 1 mg/mL
- Buffer
- PBS, pH 7.16
- Preservative
- Without preservative
- Storage
- -20 °C,-80 °C
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- Target
- CD14
- Alternative Name
- CD14 (CD14 Products)
- Background
- Background: biG 53 represents an excellent marker for CD14. The CD14 glycoprotein, gp 55, is present on most monocytic and macrophages like cell types: monocytes, macrophages, weekly at surface of neutrophiles like Kupffer cells, pleural phagocytic cells and dendritic reticular cells. CD14 is also observed on granulocytes and activated or transformed B-cells. Furthermore CD14 is present in a soluble form in human serum, urine and other body fluids. The CD14 Molecule has been reported to be a receptor for endotoxin. CD14 is anchored to cells by linkage to glycosylphosphatidylinositol (GPI) and functions as a high affinity receptor for LPS-LBP (lipopolysaccharide binding protein)-complexes.
- Gene ID
- 12475
- NCBI Accession
- NP_033971
- UniProt
- P10810
- Pathways
- TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades
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