LBP antibody

Catalog No. ABIN343173

Product Details anti-LBP Antibody

Target: LBP (Lipopolysaccharide Binding Protein (LBP))

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This LBP antibody is un-conjugated

Application: ELISA

Specificity: Mouse LBP. The antibody blocks LPS binding to membrane bound CD14.

Cross-Reactivity: Human, Rat (Rattus)

Purification: Protein G affinity column

Immunogen: native mouse LBP

Clone: BiG33

Isotype: IgG1

Application Details

Application Notes: ELISA ,LBP inhibition studies, Western Blot. inhibits binding of LPS to membrane bound CD14, (Inhibits LPS-binding (0.5 ug/ml) to CD14+CHO cells (2 x 106/ml) up to 1:1000, total inhibition 1:5 Binding titre at mouse LBP- ELISA: 1:50.000 Cross reacting with rat LBP

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with bidistilled water

Concentration: 1 mg/mL

Buffer: PBS, pH 7.17

Preservative: Without preservative

Storage: -20 °C,-80 °C

References for anti-LBP antibody (ABIN343173)

Dziarski, Tapping, Tobias: "Binding of bacterial peptidoglycan to CD14." in: The Journal of biological chemistry, Vol. 273, Issue 15, pp. 8680-90, (1998) (PubMed).

Weidemann, Schletter, Dziarski, Kusumoto, Stelter, Rietschel, Flad, Ulmer: "Specific binding of soluble peptidoglycan and muramyldipeptide to CD14 on human monocytes." in: Infection and immunity, Vol. 65, Issue 3, pp. 858-64, (1997) (PubMed).

Target Details for LBP

Target: LBP (Lipopolysaccharide Binding Protein (LBP))

Alternative Name: Lipopolysaccharide (LPS)-Binding Protein (LBP) (LBP Products)

Synonyms: BPIFD2 antibody, Bpifd2 antibody, Ly88 antibody, LPSBP antibody, LBP antibody, lipopolysaccharide binding protein antibody, lipopolysaccharide-binding protein antibody, LBP antibody, Lbp antibody, LOC100472839 antibody

Background: Background: Natural LBP is a 58KD glycoprotein produced in liver. It binds at lipid A of LPS with high affinity (10-9M) and reduced the cellular LPS effects at CD14+ cells (IL1ß, IL6, TNFα). It acts as opsonin for GRAM negative cells, LPS, neutrophiles and granulocytes.

Molecular Weight: ~58kDa

Gene ID: 16803

UniProt: Q61805

Pathways: TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Toll-Like Receptors Cascades, Monocarboxylic Acid Catabolic Process