CD14 antibody (AA 9-13, AA 39-44)

Catalog No. ABIN343167

Product Details anti-CD14 Antibody

Target: CD14

Binding Specificity: AA 9-13, AA 39-44

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD14 antibody is un-conjugated

Application: Flow Cytometry (FACS), ELISA, Functional Studies (Func), Blocking Peptide (BP)

Specificity: Soluble and membrane bound human CD14. The antibody blocks LPS binding to membrane bound CD14

Cross-Reactivity: Corynebacterium diphtheriae, Cow (Bovine), Horse (Equine)

Cross-Reactivity (Details): horse and bovine CD14,inhibits binding of LPS to horse and bovine CD14. Binding titre at horse CD14 1:100 and inhibition titre 1:10.

Purification: Protein A affinity column

Immunogen: Monocytes of different species and immunoaffinity purified soluble human CD14

Clone: BiG10

Isotype: IgG1

Application Details

Application Notes: total inhibition of LPS-binding to CD14: 100 μg/mL. Binding titre at human CD14 transfected CHO-cells: more than 10.000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with bidistilled water

Concentration: 1 mg/mL

Buffer: PBS, pH 7.4

Preservative: Without preservative

Storage: -20 °C,-80 °C

References for anti-CD14 antibody (ABIN343167)

Hackett, Flaminio, Fortier: "Analysis of CD14 Expression Levels in Putative Mesenchymal Progenitor Cells Isolated from Equine Bone Marrow." in: Stem cells and development, (2010) (PubMed).

Mérant, Breathnach, Kohler, Rashid, Van Meter, Horohov: "Young foal and adult horse monocyte-derived dendritic cells differ by their degree of phenotypic maturity." in: Veterinary immunology and immunopathology, Vol. 131, Issue 1-2, pp. 1-8, (2009) (PubMed).

Stelter, Pfister, Bernheiden, Jack, Bufler, Engelmann, Schuett: "The myeloid differentiation antigen CD14 is N- and O-glycosylated. Contribution of N-linked glycosylation to different soluble CD14 isoforms." in: European journal of biochemistry / FEBS, Vol. 236, Issue 2, pp. 457-64, (1996) (PubMed).

Target Details for CD14

Target: CD14

Alternative Name: CD14 (CD14 Products)

Synonyms: CD14 molecule antibody, CD14 antigen antibody, CD14 antibody, Cd14 antibody

Background: Background: biG 10 represents an excellent marker for CD14. The CD14 glycoprotein, gp 55, is present on most monocytic and macrophages like cell types: monocytes, macrophages, weekly at surface of neutrophiles like Kupffer cells, pleural phagocytic cells and dendritic reticular cells. CD14 is also observed on granulocytes and activated or transformed B-cells. Furthermore CD14 is present in a soluble form in human serum, urine and other body fluids. The CD14 Molecule has been reported to be a receptor for endotoxin. CD14 is anchored to cells by linkage to glycosylphosphatidylinositol (GPI) and functions as a high affinity receptor for LPS-LBP (lipopolysaccharide binding protein)-complexes.

Molecular Weight: ~50kDa

Gene ID: 929

NCBI Accession: NP_000582

UniProt: P08571

Pathways: TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades