ERAP2 antibody (AA 480-493)

Catalog No. ABIN343047

Product Details anti-ERAP2 Antibody

Target: ERAP2 (Endoplasmic Reticulum Aminopeptidase 2 (ERAP2))

Binding Specificity: AA 480-493

Reactivity: Human, Monkey

Host: Goat

Clonality: Polyclonal

Conjugate: This ERAP2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Specificity: Human ERAP2 / Aminopeptidase.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey (100%) Pig (93%) Marmoset, Elephant, Dog, Bat, Bovine, Horse (86%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-QYLKKFSYRNAKND from an internal region of human ERAP2 / Aminopeptidase (NP_071745.1). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey (100%), Pig (93%), Marmoset, Elephant, Dog, Bat, Bovine, Horse (86%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: Peptide-ELISA (1:16000, 0.5 - 1.5 μg/mL), WB (0.5 - 1.5 μg/mL)

Usage: Peptide ELISA: antibody detection limit dilution 1:16000. Western Blot: Approx. 110kD band observed in Human Spleen lysates (calculated MW of 110kD according to NP_071745.1). Recommended concentration: 0.5-1.5 μg/mL. An additional band of unknown identity was also consistently observed at 50kD. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies / lysates? Have any further splice variants / modified forms been reported?

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for ERAP2

Target: ERAP2 (Endoplasmic Reticulum Aminopeptidase 2 (ERAP2))

Abstract: ERAP2 Products

Synonyms: L-RAP antibody, LRAP antibody, ERAP2 antibody, zgc:172163 antibody, endoplasmic reticulum aminopeptidase 2 antibody, ERAP2 antibody, erap2 antibody

Background: Name/Gene ID: ERAP2
Subfamily: Metallopeptidase M1
Family: Exopeptidase

Synonyms: ERAP2, Aminopeptidase, L-RAP, LRAP

Gene ID: 64167

NCBI Accession: NP_071745

UniProt: Q6P179