BRK1 antibody (C-Term)

Catalog No. ABIN341474

Product Details anti-BRK1 Antibody

Target: BRK1 (BRICK1, SCAR/WAVE Actin-Nucleating Complex Subunit (BRK1))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio), Dog, Pig, Cow, Horse, Monkey, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BRK1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: Human C3orf10

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Bovine, Xenopus, Pike, Catfish, Salmon, Sablefish, Zebrafish (100%) Tick (92%) Drosophila, Mosquito (85%).

Purification: Protein A purified

Immunogen: Synthetic peptide from C-Terminus of human BRK1 (Q8WUW1, NP_060932). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bovine, Horse, Pig, Opossum, Xenopus, Seabass, Catfish, Salmon, Sablefish, Stickleback, Pike, Zebrafish (100%), Drosophila (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P, WB

Usage: ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 2.5 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for BRK1

Target: BRK1 (BRICK1, SCAR/WAVE Actin-Nucleating Complex Subunit (BRK1))

Alternative Name: C3orf10 (BRK1 Products)

Background: Name/Gene ID: BRK1

Synonyms: BRK1, C3orf10, MDS027, HHBrk1, Probable protein BRICK1, Protein BRICK1, HSPC300

Gene ID: 55845

NCBI Accession: NP_060932

UniProt: Q8WUW1

Pathways: RTK Signaling