West Nile Virus antibody

Catalog No. ABIN340476

Product Details anti-West Nile Virus Antibody

Target: West Nile Virus (WNV)

Reactivity: West Nile Virus (WNV)

Host: Mouse

Clonality: Monoclonal

Conjugate: This West Nile Virus antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunoprecipitation (IP), Neutralization (Neut)

Specificity: Recognizes West Nile Virus NS2b subunit.

Purification: Protein G purified

Immunogen: E. coli-derived rwnvNS3/NS2b.

Type of Immunogen: Recombinant protein

Clone: 345712

Isotype: IgG3

Application Details

Application Notes: Approved: ELISA, IP, Neut, WB

Usage: Western Blot, Neutralization, Immunoprecipitation, Direct ELISA. Western Blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect west nile virus NS3/NS2b complex. Using a colorimetric detection system, the detection limit for rwnvNS3/NS2b complex is approximately 5 ng/lane under non-reducing and reducing conditions. Chemiluminescent detection will increase sensitivity by 5 to 50 fold. Neutralization of west nile virus NS3 bioactivity: This antibody has been used to inhibit/neutralize the enzymatic activity of rwnvNS3/NS2b. The antibody was preincubated with the enzyme at different molar ratios at room temperature for 30 min. The enzyme was then assayed with a peptide substrate, Ac-REKR-AFC. 50 % of the proteolytic activity was inhibited by the antibody at approximately 13 μg/mL (IC50) under conditions in which the enzyme was present at 10 μg/mL and the substrate concentration was 200uM. Considering the molecular masses of the enzyme (27kD) and the antibody (150kD), IC50 was achieved at approximately 1:4 Molar ratio of the antibody to the enzyme. Immunoprecipitation: This antibody was used at a concentration of 25 μg/mL to immunoprecipitate rwnvNS3/NS2b from conditioned cell culture medium. The recovered rwnvNS3/NS2b can be detected in Western blots using a polyclonal antibody. Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect west nile virus NS3/NS2b complex. The detection limit for rwnvNS3/NS2b is approximately 1 ng/well.

Comment:

Target Species of Antibody: West Nile Virus

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitution: Reconstitute, sterile TBS. If 1 mL of TBS is used, the antibody concentration will be 100 μg/mL.

Concentration: Lot specific

Buffer: Lyophilized from TBS, 5 % trehalose. Reconstitution: Reconstitute, sterile TBS. If 1 mL of TBS is used, the antibody concentration will be 100 μg/mL.

Target Details for West Nile Virus

Target: West Nile Virus (WNV)

Alternative Name: West Nile Virus (WNV Products)

Target Type: Virus