Phosphoserine antibody

Catalog No. ABIN340085

Product Details anti-Phosphoserine Antibody

Target: Phosphoserine

Reactivity: Please inquire

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Phosphoserine antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunohistochemistry (IHC)

Specificity: Recognizes phosphoserine, peptidyl-phosphoserine and serine-phosphorylated proteins. Does not cross-react with ATP, phosphotyrosine, peptidyl phosphothreonine and serine. Slight cross-reactivity with free phosphothreonine. Readily reacts to known phosphoproteins such as phosvitin and alpha casein. Species cross-reactivity: All species.

Purification: Immunoaffinity purified

Immunogen: Phosphoserine conjugated with KLH.

Type of Immunogen: Hapten

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (1:250 - 1:1000), IHC (1:50), IP, WB

Usage: Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and ELISA. Western Blot (tissue lysates): 50-100 μg/lane total protein, antibody dilution can be from 1:250-1:1000, overloading will show solid exposed lanes. Dilute the primary antibody until multiple phosphorylated bands will appear. If using purified proteins dilutions from 1:50-1:1000 will need to be tried. At 1:300 dilution the antibody normally detects 10 ng of protein with 2 hour incubations and enhanced HRP detection. Blocking of the blot should be with non-phosphoserine containing compounds such as 1-5 % BSA in TBS that has been filtered through a 0.45um. Milk or casein is not recommended because of the presence of phospho-serine in these solutions. Buffers should be Tris based so that phosphate ion interference is minimized. Immunoprecipitation (tissue extracts): 10-20 μg/mg protein. Note that this will immunoprecipitate all phosphoserine proteins in the extracts. If using purified protein exacts containing only the protein of interest, antibody amounts should be decreased to 5 μg/500 μL of extracts. ELISA (kinase assay): 1:250-1:500. Immunohistochemistry: 1:50. Detects all phosphoserines, and any phosphorylated serine protein or peptide can be used to block antibody staining. Typically, a10M excess of peptide is used in Tris based buffers. Positive control: NIH 3T3 cells (+/- TPA), K562 cells and EGF-stimulated A431 cells.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 50 % glycerol.

Handling Advice: Avoid repeated freezing and thawing

Storage: -20 °C

Storage Comment: For long-term storage and to avoid freeze-thaw cycles, aliquot and store at -20°C. Aliquots are stable for at least 1 year at -20°C.

Target Details for Phosphoserine

Target: Phosphoserine

Abstract: Phosphoserine Products

Target Type: Amino Acid