Recognizes and is specific for recombinant proteins containing the Myc epitope tag (EQKLISEEDL) in a variety of sequence contexts. Also recognizes human Myc. Species cross-reactivity: Human. Other species cross-reactivity not tested.
Predicted Reactivity
Percent identity by BLAST analysis: Mouse (100%) Human, Chimpanzee, Gorilla, Rat, Amoeba (91%) Orangutan, Monkey, Marmoset, Tamarin, Panda, Cat (82%).
Purification
Protein G purified
Immunogen
KLH-conjugated, synthetic peptide corresponding to amino acids 410-420 (MEQKLISEEDL) of human Myc. Percent identity by BLAST analysis: Mouse (100%), Human, Chimpanzee, Gorilla, Rat, Amoeba (91%), Orangutan, Monkey, Marmoset, Tamarin, Panda, Cat (82%).
Type of Immunogen: Synthetic peptide - KLH conjugated
Usage: Suitable for use in Western Blot. Western Blot: 0.1-1 μg/mL detects Myc-tagged recombinant protein. COS transfected cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-myc tag, biotin conjugate (1 μg/mL). Proteins were visualized using a streptavidin-HRP conjugate and a chemiluminescence detection system. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Restrictions
For Research Use only
Format
Liquid
Concentration
Lot specific
Buffer
PBS, 0.05 % sodium azide, 30 % glycerol.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.