AKT Substrate (pSer), (pThr) antibody
ELISA, WB, IHC (p), IP
Host: Rabbit
Polyclonal
unconjugated
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- Target
- AKT Substrate
- Binding Specificity
- pSer, pThr
- Reactivity
- Please inquire
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Host
- Rabbit
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Clonality
- Polyclonal
-
Conjugate
- Un-conjugated
- Application
- ELISA, Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
- Specificity
- Preferentially recognizes peptides and proteins containing phospho-Ser/Thr preceded by Lys/Arg at positions -5 and -3, in a manner largely independent of other surrounding amino acids. Some cross-reactivity is observed for peptides that contain phospho-Ser/Thr preceded by Arg/Lys at positions -3 and -2. No cross-reactivity is observed with the corresponding non-phosphorylated sequences or with other phospho-Ser/Thr-containing motifs. By ELISA the antibody recognizes a wide range of phosphorylated Akt substrate peptides and by 2D gel Western blot analysis it recognizes a large number of presumptive Akt substrate proteins.
- Purification
- Immunoaffinity Chromatography
- Immunogen
- Synthetic phospho- Akt substrate peptides.
- Isotype
- IgG
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- Application Notes
- ELISA (1:1000), IHC-P, IP (1:20), WB (1:1000)
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- 10 mM sodium HEPES, pH 7.5, 150 mM sodium chloride, BSA, 50 % glycerol.
- Handling Advice
- avoid freeze thaw cycles. Store undiluted.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted.
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- Target
- AKT Substrate
- Target Type
- Chemical
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