Recognizes GABA (gamma-aminobutyric acid). The antibody has been calibrated against a spectrum of antigens to assure hapten selectivity and proper affinity. No measurable glutaraldehyde-fixed tissue cross-reactivity (<1:1000) against L-alanine, 1-amino-4-guanidobutane (AGB), D/L-arginine, D/L-aspartate, L-citrulline, L-cysteine, D/L-glutamate, D/L-glutamine, glutathione, glycine, Lysine, L-ornithine, L-serine, L-threonine, L-tryptophan, L-tyrosine.
GABA
WB, IHC, ICC, IF
Host: Rabbit
Polyclonal
FITC
Application Notes
Approved: ELISA, IF, IHC, IHC-Fr
Usage: Suitable for use in ELISA, Immunofluorescence and Immunohistochemistry. Immunohistochemistry: 1:100 using silver-intensified immunogold or fluorescence. Samples should be fixed with 0.05-2.5 % glutaraldehyde for optimum detection. This antibody has also been used and found to work with a zero-low glutaraldehyde/high paraformaldehyde fixation (4 % paraformaldehyde in 0.1 M phosphate buffer/3 % sucrose fixative). The minimum glutaraldehyde concentration is 0 %. Performance is good with frozen sections, Vibratome sections and tissue culture formats, when penetrating reagents such as 0.3 % Triton X-100 are used.