COPB2 antibody (AA 494-511)

Catalog No. ABIN337394

Product Details anti-COPB2 Antibody

Target: COPB2 (Coatomer Protein Complex, Subunit beta 2 (COPB2))

Binding Specificity: AA 494-511

Reactivity: Human, Mouse, Rat, Monkey, Pig, Bat, Cow, Dog, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This COPB2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Detects rat coatomer-protein I beta (COP I beta).

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bat, Bovine, Horse, Pig, Opossum, Platypus (100%) Orangutan, Rabbit, Lizard (94%) Chicken (89%) Turkey, Xenopus, Sea anemone (83%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide: KVLAAQETHEGVTEDGIE, corresponding to amino acids 494-511 of Rat beta COP I. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bat, Bovine, Horse, Pig, Opossum, Platypus (100%), Orangutan, Rabbit, Lizard (94%), Chicken (89%), Turkey, Xenopus, Sea anemone (83%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: 2 μg/mL, Detects an ~115kD protein representing COP I beta from PC-12 cell extract.

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 1 mg/mL BSA, 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Target Details for COPB2

Target: COPB2 (Coatomer Protein Complex, Subunit beta 2 (COPB2))

Alternative Name: Beta-COP / COPB2 (COPB2 Products)

Background: Name/Gene ID: COPB2

Synonyms: COPB2, Betaprime-COP, Beta'-COP, Beta-coat protein, Beta-COP, p102, Coatomer beta' subunit, Coatomer subunit beta

Gene ID: 9276

UniProt: P35606