GLUL antibody

Catalog No. ABIN337301

Product Details anti-GLUL Antibody

Target: GLUL (Glutamate-Ammonia Ligase (GLUL))

Reactivity: Human, Rat, Sheep

Host: Mouse

Clonality: Monoclonal

Conjugate: This GLUL antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Recognizes sheep Glutamine Synthase. Species cross-reactivity: rat.

Purification: Protein A purified

Immunogen: Glutamine synthetase purified from sheep brain.

Type of Immunogen: Purified protein

Clone: GS-6

Isotype: IgG2a

Application Details

Application Notes: Approved: ELISA, IHC, IHC-P (10 μg/mL), WB (1:1000 - 1:5000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. Western Blot: 1:500. Single band in sheep and rat brain. Positive control: Rat brain tissue, rat brain cytosolic fraction, human brain lysate.

Comment:

Target Species of Antibody: Sheep

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.5, 0.09 % sodium azide, BSA, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for GLUL

Target: GLUL (Glutamate-Ammonia Ligase (GLUL))

Alternative Name: GLUL / Glutamine Synthetase (GLUL Products)

Background: Name/Gene ID: GLUL

Synonyms: GLUL, GLNS, Glutamate decarboxylase, Glutamate-ammonia ligase, Glutamine synthase, GS, Glutamate--ammonia ligase, Glutamine synthetase, PIG43, PIG59

Gene ID: 2752

UniProt: P15104

Pathways: Positive Regulation of Peptide Hormone Secretion