COPS5 antibody (Internal Region)

Catalog No. ABIN337275

Product Details anti-COPS5 Antibody

Target: COPS5 (COP9 Constitutive Photomorphogenic Homolog Subunit 5 (Arabidopsis) (COPS5))

Binding Specificity: Internal Region

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This COPS5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Brand: IHC-plus™

Specificity: Recognizes human JAB1 protein at ~40kD. Reactivity has been confirmed with human 293T and Jurkat cell lysates by Western Blot and immunoprecipitation.

Purification: Protein A purified

Immunogen: Synthetic peptide derived from the internal region of JAB1 (Jun activation domain-binding protein 1, CSN5).

Type of Immunogen: Synthetic peptide

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: IHC, IHC-P (10 μg/mL), IP (1 - 3 μg/mL), WB (1 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Target Details for COPS5

Target: COPS5 (COP9 Constitutive Photomorphogenic Homolog Subunit 5 (Arabidopsis) (COPS5))

Alternative Name: COPS5 / JAB1 (COPS5 Products)

Background: Name/Gene ID: COPS5

Synonyms: COPS5, 38 kDa Mov34 homolog, JAB1, Signalosome subunit 5, MOV-34, SGN5, CSN5

Gene ID: 10987

UniProt: Q92905

Pathways: Cell Division Cycle