PEDF antibody (AA 196-347)

Catalog No. ABIN337197

Product Details anti-PEDF Antibody

Target: PEDF (SERPINF1) (serpin Peptidase Inhibitor, Clade F (Alpha-2 Antiplasmin, Pigment Epithelium Derived Factor), Member 1 (SERPINF1))

Binding Specificity: AA 196-347

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PEDF antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Brand: IHC-plus™

Specificity: Recognizes human GST-PEDF fusion protein with a MW of ~46kD.

Purification: Protein A purified

Immunogen: Recombinant human GST-PEDF fusion protein. The sequence corresponding to aa196-347 of human PEDF tagged with GST on the N-terminal was expressed in E. coli. GST-PEDF is about 46kD on SDS-PAGE.

Type of Immunogen: Recombinant protein

Isotype: IgG2a

Application Details

Application Notes: Approved: ELISA, ICC, IHC, IHC-Fr, IHC-P (5 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.6, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid freeze-thaw cycles.

Target Details for PEDF

Target: PEDF (SERPINF1) (serpin Peptidase Inhibitor, Clade F (Alpha-2 Antiplasmin, Pigment Epithelium Derived Factor), Member 1 (SERPINF1))

Alternative Name: SERPINF1 / PEDF (SERPINF1 Products)

Synonyms: EPC-1 antibody, OI12 antibody, OI6 antibody, PEDF antibody, MGC82984 antibody, SERPINF1 antibody, AI195227 antibody, Pedf antibody, Pedfl antibody, Sdf3 antibody, Dmrs91 antibody, zgc:92072 antibody, serpin family F member 1 antibody, serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1 antibody, serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1 L homeolog antibody, serine (or cysteine) peptidase inhibitor, clade F, member 1 antibody, SERPINF1 antibody, serpinf1 antibody, serpinf1.L antibody, Serpinf1 antibody

Background: Name/Gene ID: SERPINF1

Synonyms: SERPINF1, EPC-1, OI12, PEDF, Serpin F1, PIG35, OI6

Gene ID: 5176

UniProt: P36955