DUSP1 antibody (C-Term)

Catalog No. ABIN337195

Product Details anti-DUSP1 Antibody

Target: DUSP1 (Dual Specificity Phosphatase 1 (DUSP1))

Binding Specificity: AA 87-101, C-Term

Reactivity: Human, Rat, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DUSP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Recognizes human MKP1, Mr ~42kD. Species cross-reactivity: mouse. Predicted to cross-react with rat based on sequence homology.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Marmoset, Rat, Dog (100%) Monkey, Mouse, Panda, Bovine, Rabbit (93%) Hamster, Pig (87%) Elephant (80%).

Purification: Protein A purified

Immunogen: KLH-conjugated, synthetic peptide corresponding to amino acids 87-101 (DERSAALDGAKRDGT-C) of human MAP kinase phosphatase 1 (MKP1), with a C-terminal cysteine added for conjugation purposes. The immunizing sequence is identical in rat and mouse. Percent identity by BLAST analysis: Human, Gorilla, Marmoset, Rat, Dog (100%), Monkey, Mouse, Panda, Bovine, Rabbit (93%), Hamster, Pig (87%), Elephant (80%).

Type of Immunogen: Synthetic peptide - KLH conjugated

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.1 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: 0.1-1 μg/mL detects MKP1 in RIPA lysates from 3T3/A31 cells. 3T3/A31 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-MKP1 (0.3 μg/mL).

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide, before the addition of glycerol to 30 %

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for DUSP1

Target: DUSP1 (Dual Specificity Phosphatase 1 (DUSP1))

Alternative Name: MKP-1 / DUSP1 (DUSP1 Products)

Background: Name/Gene ID: DUSP1
Subfamily: Dual specificity MKP
Family: Protein Phosphatase

Synonyms: DUSP1, 3CH134, Dual specificity phosphatase 1, ERK-inactivating phosphatase, HVH1, MAP kinase phosphatase 1, VH1, PTPN10, CL100, MKP-1, MKP1

Gene ID: 1843

UniProt: P28562