STAT5 A/B antibody (pTyr694)

Catalog No. ABIN337157

Product Details anti-STAT5 A/B Antibody

Target: STAT5 A/B

Binding Specificity: pTyr694

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This STAT5 A/B antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: A BLAST analysis was used to suggest cross-reactivity with Stat5 alpha from human, mouse and rat based on 100 % homology with the immunizing sequence. Cross-reactivity with Stat5 alpha from other sources has not been determined.

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide corresponding to residues surrounding Y694 of mouse STAT5 alpha protein.

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: ELISA (1:25000), IHC, IHC-P (20 - 40 μg/mL), WB (1:500 - 1:2000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 20-40 μg/mL. Western Blot: Expect a band approximately 91 kD in size corresponding to phosphorylated Stat5a protein in the appropriate cell lysate or extract. This phosphospecific polyclonal antibody reacts with mouse Stat5a pY694 and shows minimal reactivity by ELISA against the nonphosphorylated form of the immunizing peptide.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 0.01 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for STAT5 A/B

Target: STAT5 A/B

Alternative Name: STAT5A+B (STAT5 A/B Products)

Background: This antibody detects: STAT5 A+B