VPS25 antibody (N-Term)

Catalog No. ABIN337136

Product Details anti-VPS25 Antibody

Target: VPS25 (Vacuolar Protein-Sorting-Associated Protein 25 (VPS25))

Binding Specificity: N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This VPS25 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Synthetic peptide corresponding to N-terminal residues of human VPS25(Vacuolar protein sorting protein 25).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from human VPS25.

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: ELISA, IHC, IHC-P (5 μg/mL), WB (1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.01 % sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for VPS25

Target: VPS25 (Vacuolar Protein-Sorting-Associated Protein 25 (VPS25))

Alternative Name: VPS25 (VPS25 Products)

Background: Name/Gene ID: VPS25

Synonyms: VPS25, DERP9, EAP20, ESCRT-II complex subunit VPS25, FAP20, HVps25

Gene ID: 84313

UniProt: Q9BRG1